Ursolic Acid Induces Apoptotic Cell Death Through AIF and Endo G Release Through a Mitochondria-dependent Pathway in NCI-H292 Human Lung Cancer Cells
| Autor: | Y. Y. Shih, Jiann-Shang Chou, Ming-Yang Yeh, Nien-Chieh Liao, Mei-Hui Lee, Hsueh-Yu Chung, Jing Gung Chung, Ko-Lin Liu, Hsin-Yu Hou, Pei-Yi Chou, Chiung-Ju Chen |
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| Rok vydání: | 2018 |
| Předmět: |
Cancer Research
Lung Neoplasms Apoptosis DNA Fragmentation Mitochondrion General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ursolic acid Annexin Cell Line Tumor Cytotoxic T cell Humans Propidium iodide Viability assay Cell Proliferation Pharmacology Membrane Potential Mitochondrial Cell Cycle Checkpoints Molecular biology Triterpenes Mitochondria chemistry 030220 oncology & carcinogenesis DNA fragmentation Signal Transduction Research Article |
| Zdroj: | In vivo (Athens, Greece). 33(2) |
| ISSN: | 1791-7549 |
| Popis: | Background/Aim: Ursolic acid (UA), a triterpene compound present in natural plants, has been shown to induce cytotoxic effects on many human cancer cells through induction of cell-cycle arrest and apoptosis. This study investigated the effects of UA on human lung cancer NCI-H292 cells in vitro. Materials and Methods: Flow cytometric assay was used to measure the percentage of cell viability, apoptotic cell death by double staining of annexin V and propidium iodide (PI), production of reactive oxygen species (ROS) and Ca(2+), and mitochondriaI membrane potential (Ψ(m)). UA-induced chromatin condensation and DNA fragmentation were examined by 4’,6-diamidino-2-phenylindole staining and DNA gel electrophoresis, respectively. Western blotting was used to examine the changes of apoptosis-associated protein expression in NCI-H292 cells. Results: UA reduced cell viability and induced apoptotic cell death. UA increased Ca(2+) production, reduced Ψ(m), but did not affect ROS production in NCI-H292 cells. UA increased apoptosis-inducing factor (AIF) and endonuclease G in NCI-H292 cells. Conclusion: Based on these observations, we suggest UA induces apoptotic cell death via AIF and Endo G release through a mitochondria-dependent pathway in NCI-H292 cells. |
| Databáze: | OpenAIRE |
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