Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine
| Autor: | Roberto Lamberti, Luis Sepulveda, Eduardo Herrero, Marcos Arezo, Claudia Grizmado, Leonardo Molina, Marshall W. Lightowlers, Katherina A. Vizcaychipi, Arnoldo Calabro, Meritxell Donadeu, Daniel Araya, Jose Luis Labanchi, Guillermo Mujica, Lilia Mabel Gino, Mariela Alejandra García Cachau, Gabriel Talmon, Marcos Seleiman, Edmundo Larrieu, Graciela Santillán, Charles G. Gauci, Pablo Crowley, Thelma Veronica Poggio |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
VACCINE EG95 CIENCIAS MÉDICAS Y DE LA SALUD 030231 tropical medicine Antibodies Helminth Argentina Sheep Diseases Enzyme-Linked Immunosorbent Assay Pilot Projects Biotecnología de la Salud Cohort Studies 03 medical and health sciences Dogs 0302 clinical medicine Echinococcosis Animals Humans Medicine Analysis of Variance Vaccines Sheep Echinococcus granulosus Immunization Programs business.industry Cystic echinococcosis medicine.disease Virology Immunity Humoral HUMORAL RESPONSE 030104 developmental biology Infectious Diseases SHEEP Immunoglobulin G ECHINOCOCCOSIS Ethnology Parasitology business Otras Biotecnologías de la Salud |
| Zdroj: | Parasitology International. 66:258-261 |
| ISSN: | 1383-5769 |
| Popis: | Cystic echinococcosis is endemic in the Rio Negro province of Argentina and, for this, a control program using praziquantel in dogs was developed from 1980. The transmission rate to humans and sheep has decreased significantly, however transmission persists. In 2009 the vaccination of sheep with EG95 was incorporated in some areas of the province. The objective of the study was to evaluate the humoral responses to the vaccine EG95. Lambs received two vaccinations with the EG95 vaccine followed by a single booster injection when the animals were 1-1.5 years of age. Blood samples from 6 vaccinated groups and 4 no vaccinated sheep were obtained for determination of antibody titles against EG95 protein. Anti-EG95 responses were determined as described by Heath and Koolaard, 2012. Responses were evaluated from 331 animals. Median ELISA absorbance values in vaccinated group was 0.828, and in non-vaccinated groups was 0.218. EG95 antibody responses in sheep from different cohorts of non-immunized control groups and vaccinated groups reveal the sustained increase in response seen in animals following the third immunization. (p< 0.0001). Significant differences are also evidenced in ANOVA test (p< 0.001). An anti-EG95 antibody response was induced in all groups of immunized sheep, 4 times higher (0.828) than the mean observed in the control groups (0.218). Data described here indicate that following a third vaccination with the EG95 vaccine at 1 year of age the specific IgG responses detected in the serum of sheep increased to a level greater than that seen following the second immunization and that this response was maintained longitudinally over time, for at least 5 years. Fil: Larrieu, Edmundo Juan. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Negro; Argentina Fil: Poggio, Thelma Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina Fil: Mujica, Guillermo. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: Gauci, Charles G.. University of Melbourne; Australia Fil: Labanchi, Jose Luis. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: Herrero, Eduardo. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: Araya, Daniel. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: Grizmado, Claudia. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: Calabro, Arnoldo. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: Talmon, Gabriel. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: Crowley, Pablo Ernesto. Universidad Nacional de Río Negro; Argentina Fil: Santillán, Graciela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina Fil: Vizcaychipi, Katherina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina Fil: Seleiman, Marcos. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: Sepulveda, Luis. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: Arezo, Marcos. Provincia de Río Negro. Ministerio de Salud; Argentina Fil: García Cachau, Mariela Alejandra. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina Fil: Lamberti, Roberto. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina Fil: Molina, Leonardo. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina Fil: Gino, Lilia Mabel. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina Fil: Donadeu, Meritxell. University of Melbourne; Australia Fil: Lightowlers, Marshall W.. University of Melbourne; Australia |
| Databáze: | OpenAIRE |
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