Leukotriene C4 formation by enriched human basophil preparations from normal and asthmatic subjects
| Autor: | E. Van Donk, W. Blok, P.L.B. Bruijnzeel, P.T.M Kok |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
education Clinical Biochemistry Prostaglandin chemical and pharmacologic phenomena Stimulation Basophil Histamine Release chemistry.chemical_compound Internal medicine parasitic diseases medicine Humans Calcimycin Leukotriene Leukotriene C4 Prostaglandin D2 Zymosan hemic and immune systems Cell Biology Immunoglobulin E Opsonin Proteins Asthma Antibodies Anti-Idiotypic Basophils Endocrinology medicine.anatomical_structure chemistry SRS-A Histamine |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids. 44:11-17 |
| ISSN: | 0952-3278 |
| DOI: | 10.1016/0952-3278(91)90138-u |
| Popis: | Numbers of circulating basophils are increased in asthmatic subjects, compared to normal subjects. Basophil enriched cell preparations from normal and asthmatic subjects were challenged in vitro with the calcium ionophore A23187, anti-IgE, or opsonized zymosan to study leukotriene C 4 formation, histamine release, and prostaglandin D 2 formation. No prostaglandin D 2 formation by basophils was observed. Furthermore, opsonized zymosan was not capable of inducing any mediator formation or release from basophils. At optimal stimulation conditions no differences were found between basophils from normal and asthmatic subjects concerning A23187 or anti-IgE induced leukotriene C 4 formation or histamine release. A23187 and anti-IgE induced leukotriene C 4 formation were in the range of 1–20 and 0.6–4.8 pmol/10 6 basophils respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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