Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease
| Autor: | O. John Semmes, Julius O. Nyalwidhe, Robert Given, Elena Igwe, Raymond S. Lance, Krista Y. White, Richard R. Drake, Thomas W. Fuller, Mary Ann Clements |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Proteomics PCA3 Prostatic Diseases medicine.medical_specialty Time Factors medicine.medical_treatment Acid Phosphatase Biophysics Urology Biology Biochemistry Article Prostate cancer Prostate medicine Humans Electrophoresis Gel Two-Dimensional Glycomics Prostate massage Prostatectomy Prostatic Neoplasms Epithelial Cells medicine.disease Body Fluids Gene Expression Regulation Neoplastic Prostate-specific antigen medicine.anatomical_structure Prostatic acid phosphatase Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Immunology Protein Tyrosine Phosphatases Biomarkers |
| Zdroj: | Journal of Proteomics. 72:907-917 |
| ISSN: | 1874-3919 |
| DOI: | 10.1016/j.jprot.2009.01.007 |
| Popis: | The prostate gland secretes many proteins in a prostatic fluid that combines with seminal vesicle derived fluids to promote sperm activation and function. Proximal fluids of the prostate that can be collected clinically are seminal plasma and expressed-prostatic secretion (EPS) fluids. EPS represents the fluid being secreted by the prostate following a digital rectal prostate massage, which in turn can be collected in voided urine post-exam. This collection is not disruptive to a standard urological exam, and it can be repeatedly collected from men across all prostatic disease states. A direct EPS fluid can also be collected under anesthesia prior to prostatectomy. While multiple genetic assays for prostate cancer detection are being developed for the shed epithelial cell fraction of EPS urines, the remaining fluid that contains many prostate-derived proteins has been minimally characterized. Approaches to optimization and standardization of EPS collection consistent with current urological exam and surgical practices are described, and initial proteomic and glycomic evaluations of the of EPS fluid are summarized for prostate specific antigen and prostatic acid phosphatase. Continued characterization of the prostate specific protein components of EPS urine combined with optimization of clinical collection procedures should facilitate discovery of new biomarkers for prostate cancer. |
| Databáze: | OpenAIRE |
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