Isolation, in vitro maturation, and fertilization of germinal vesicle oocytes obtained from the intact murine ovary
| Autor: | G. W. Randall, C. A. Shivers, S. G. Awadalla |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Embryology
medicine.medical_specialty Ovary Cell Separation Fertilization in Vitro Biology Chorionic Gonadotropin Human chorionic gonadotropin Follicle-stimulating hormone Mice Human fertilization Oogenesis Internal medicine Genetics medicine Animals Genetics (clinical) Cells Cultured Germinal vesicle Estradiol Obstetrics and Gynecology General Medicine Oocyte Prolactin In vitro maturation Culture Media medicine.anatomical_structure Endocrinology Reproductive Medicine Fertilization Oocytes Female Follicle Stimulating Hormone Developmental Biology |
| Zdroj: | Journal of in vitro fertilization and embryo transfer : IVF. 7(6) |
| ISSN: | 0740-7769 |
| Popis: | The purpose of this investigation was to attempt to develop a process, utilizing a murine model, which would allow more efficient harvesting from the intact ovary and maturation in vitro of germinal vesicle (GV) oocytes. The recovery process yielded 25.5±4.5 (\(\overline X \)±SE) cumulusfree GV oocytes per animal. Treatment groups included culture medium (CM) supplemented with either estradiol (E2), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), or prolactin (PRL). Among the hormone-free controls 83.2±1.6% of oocytes underwent GV breakdown, whereas 25.3±2.6% developed to the first polar body stage (PB-1) following 18 hr of incubation (n=29 trials). Oocytes progressing to the PB-1 stage were inseminated in vitro. In vitro fertilization (IVF) of pooled in vitro matured (IVM) PB-1 oocytes (judged by two-cell formation) was 19.9%, which was significantly lower than in the group of in vivo matured oocytes (74.7%). E2 significantly increased the percentage of GV breakdown (control, 76.8±2.5%; E2 at 10 ng/ml, 92.9±2.5%,P |
| Databáze: | OpenAIRE |
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