| Autor: |
Georg Widera, Marianna Foldvari, Lawrence Baizer, Maria E. Baca-Estrada, Michael Storms, Dietmar Rabussay, Richard Stout, Shawn Babiuk, Lorne A. Babiuk |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Molecular Therapy. 8:992-998 |
| ISSN: |
1525-0016 |
| DOI: |
10.1016/j.ymthe.2003.09.008 |
| Popis: |
Electroporation has been shown to increase the potency of DNA vaccines that have demonstrated significant potential in mice. However, there is a need to develop noninvasive or minimally invasive vaccination methods. In pigs, in vivo gene expression was assessed to compare intradermal needle injection to a needle-free dermal BioJect as a means of delivery of plasmids. Each administration method was further tested with and without surface electroporation. Experiments with plasmid DNA encoding luciferase demonstrated that needle-free administration results in higher gene expression levels than needle injection. Electroporation enhanced gene expression for both intradermal delivery methods. Needle-free plasmid injection in combination with electroporation led to a more rapid induction of immune responses compared to other methods of plasmid administration. It was concluded that needle-free topical electroporation significantly enhances gene expression, possibly by improving cellular uptake of plasmid DNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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