Establishment of the European meningococcal strain collection genome library (EMSC-GL) for the 2011 to 2012 epidemiological year
| Autor: | Steen Hoffmann, Carina Brehony, Alena Vaculikova, Georgina Tzanakaki, Muhamed-Kheir Taha, Dominique A. Caugant, Despo Pieridou-Bagatzouni, Jelena Galajeva, Arie van der Ende, Sigrid Heuberger, Pavla Křížová, Timea Erdosi, Marina Pana, Holly B. Bratcher, Maija Toropainen, Anna Skoczynska, Martin C. J. Maiden, Heike Claus, Metka Paragi |
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| Přispěvatelé: | University of Oxford [Oxford], National University of Ireland [Galway] (NUI Galway), Austrian Agency for Health and Food Safety (AGES), Nicosia General Hospital, National Institute of Public Health [Prague], Statens Serum Institut [Copenhagen], National Institute of Public Health and Welfare [Helsinki], Institut Pasteur [Paris], Julius-Maximilians-Universität Würzburg [Wurtzbourg, Allemagne] (JMU), National Meningitis Reference Laboratory, National School of Public Health Athens, National Center for Epidemiology - Országos Epidemiológiai Központ [Budapest] (OEK), Riga East University Hospital, University of Amsterdam [Amsterdam] (UvA), National Medicines Institute - Narodowy Instytut Leków [Warsaw] (NIL), National Institute of Research and Development for Microbiology and Immunology [Bucharest], Public Health Authority of the Slovak Republic, National Institute of Public Health of the Republic of Slovenia, Norwegian Institute of Public Health [Oslo] (NIPH), The project was funded by the ECDC within the framework of the IBD-labnet program through the Specific Contract No3 ECD.4051 implementing Framework Contract No ECDC/2011/014.Database curators are currently supported by the UK Department of Health (grant H2R00080).This publication made use of the Neisseria Multi Locus Sequence Typing website (https://pubmlst.org/neisseria/) developed by Keith Jolley and sited at the University of Oxford (Jolley & Maiden 2010, BMC Bioinformatics, 11:595). The development of this site has been funded by the Wellcome Trust and European Union, Wellcome Trust Biomedical Resource Grant (104992)., AII - Infectious diseases, Medical Microbiology and Infection Prevention, University of Oxford, Institut Pasteur [Paris] (IP), Julius-Maximilians-Universität Würzburg (JMU) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Epidemiology sequence data monitor vaccine coverage genome library Neisseria meningitidis Serogroup B Neisseria meningitidis medicine.disease_cause Genome susceptibility Genotype neisseria-meningitidis Genetics Genomic Library Molecular Epidemiology biology Strain (biology) pathogenesis Genomics 3. Good health Europe Population Surveillance surveillance serogroup-c nomenclature Neisseria population genomics 030106 microbiology Euroroundup Meningitis Meningococcal Serogroup 03 medical and health sciences Virology medicine Humans Typing Gene disease locus Whole Genome Sequencing Public Health Environmental and Occupational Health Outbreak Genetic Variation biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Meningococcal Infections Genes Bacterial Genetic Loci track antimicrobial susceptibility [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Genome Bacterial |
| Zdroj: | Eurosurveillance Eurosurveillance, European Centre for Disease Prevention and Control, 2018, 23 (20), ⟨10.2807/1560-7917.ES.2018.23.20.17-00474⟩ Euro surveillance, 23(20):17-00474. Centre Europeen pour la Surveillance Epidemiologique du SIDA Eurosurveillance, 2018, 23 (20), ⟨10.2807/1560-7917.ES.2018.23.20.17-00474⟩ |
| ISSN: | 1560-7917 1025-496X |
| DOI: | 10.2807/1560-7917.ES.2018.23.20.17-00474⟩ |
| Popis: | International audience; Invasive meningococcal disease surveillance in Europe combines isolate characterisation and epidemiological data to support public health intervention. A representative European Meningococcal Strain Collection (EMSC) of IMD isolates was obtained, and whole genome sequenced to characterise 799 EMSC isolates from the epidemiological year July 2011-June 2012. To establish a genome library (GL), the isolate information was deposited in the pubMLST.org/neisseria database. Genomes were curated and annotated at 2,429 meningococcal loci, including those defining clonal complex, capsule, antigens, and antimicrobial resistance. Most genomes contained genes encoding B (n = 525; 65.7%) or C (n = 163; 20.4%) capsules; isolates were genetically highly diverse, with >20 genomic lineages, five of which comprising 60.7% (n = 485) of isolates. There were >350 antigenic fine-types: 307 were present once, the most frequent (P1.7-2,4:F5-1) comprised 8% (n = 64) of isolates. Each genome was characterised for Bexsero Antigen Sequence Typing (BAST): 25.5% (n = 204) of isolates contained alleles encoding the fHbp and/or the PorA VR1 vaccine component, but most genomes (n = 513; 64.2%) did not contain the NadA component. EMSC-GL will support an integrated surveillance of disease-associated genotypes in Europe, enabling the monitoring of hyperinvasive lineages, outbreak identification, and supporting vaccine programme implementation. |
| Databáze: | OpenAIRE |
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