BDNF Injected into the Superior Colliculus Reduces Developmental Retinal Ganglion Cell Death
| Autor: | Ted Hsieh, M E Forbes, Douglas O. Frost, Yun-Tao Ma, James E. Johnson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Retinal Ganglion Cells
medicine.medical_specialty Superior Colliculi Time Factors genetic structures medicine.medical_treatment Amidines Biology Retinal ganglion Axonal Transport Article Retina Injections Neurotrophic factors Internal medicine Cricetinae medicine Animals Fluorescent Dyes Cell Death Mesocricetus General Neuroscience Superior colliculus Brain-Derived Neurotrophic Factor biology.organism_classification eye diseases medicine.anatomical_structure Endocrinology Retinal ganglion cell nervous system biology.protein sense organs Axotomy Neuroscience Pyknosis Neurotrophin |
| Popis: | The role of neurotrophins as survival factors for developing CNS neurons, including retinal ganglion cells (RGCs), is uncertain. Null mutations for brain-derived neurotrophic factor (BDNF) or neurotrophin 4 (NT4), individually or together, are without apparent effect on the number of RGCs that survive beyond the period of normal, developmental RGC death. This contrasts with the BDNF dependence of RGCsin vitroand the effectiveness of BDNF in reducing RGC loss after axotomy. To investigate the effect of target-derived neurotrophins on the survival of developing RGCs, we injected BDNF into the superior colliculus (SC) of neonatal hamsters. At the age when the rate of developmental RGC death is greatest, BDNF produces, 20 hr after injection, a 13–15-fold reduction in the rate of RGC pyknosis compared with the rates in vehicle-injected and untreated hamsters. There is no effect 8 hr after injection. Electrochemiluminescence immunoassay measurements of BDNF protein in the retinae and SC of normal and BDNF-treated hamsters demonstrate that the time course of BDNF transport to RGCs supports a role for target-derived BDNF in promoting RGC survival. The effectiveness of pharmacological doses of BDNF in reducing developmental RGC death may be useful in further studies of the mechanisms of stabilization and elimination of immature central neurons. |
| Databáze: | OpenAIRE |
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