Cytokine-induced tumor immunogenicity: endogenous interleukin-1 alpha expressed by fibrosarcoma cells confers reduced tumorigenicity
| Autor: | Tatyana Dvorkin, Mahmoud Huleihel, Daniel Benharroch, Moshe Hacham, Elena Voronov, Noa Shimoni, Amos Douvdevani, Eyal Fima, Margot Zöller, Ron N. Apte, Shraga Segal, Rosalyn M. White |
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| Rok vydání: | 1993 |
| Předmět: |
Stromal cell
medicine.medical_treatment T cell Fibrosarcoma Immunology Antigen presentation Mice Nude Biology Transfection Mice medicine Immunology and Allergy Animals Remission Induction Immunotherapy 3T3 Cells medicine.disease Cytokine medicine.anatomical_structure Cell Transformation Neoplastic Cell culture Cancer research Tumor necrosis factor alpha Interleukin-1 |
| Zdroj: | Immunology letters. 39(1) |
| ISSN: | 0165-2478 |
| Popis: | A direct correlation between the constitutive expression of IL-1α and reduced tumorigenicity of fibrosarcomas was observed. This was established in fibrosarcoma cell lines which produce IL-1α ‘spontaneously’, possibly as an aberration of oncogene-mediated transformation or upon IL-1α gene transfer. In fibroblasts intracellular or membrane-associated IL-1α is expressed, whereas the secreted form of the cytokine (IL-1β) is absent. Studies on the mechanisms of tumor regression of the IL-1α-positive fibroblastoid cell lines indicated that IL-1α potentiates the development of tumor cell-specific CTLs, which are of importance for tumor eradication. Thus, IL-1α induces enhanced helper T cell activity which provides auxiliary signals for the growth/development of CTLs. Non-adaptive effector cells, activated locally by IL-1α-expressing fibrosarcoma cells, also contribute to the eradication of IL-1α-expressing fibrosarcomas. Local IL-1α expression potentiated antigen presentation, by the malignant fibroblasts as well as by tissue-resident antigen-presenting cells, thus further potentiating anti-tumor immune responses. Mice, in which IL-1α-producing tumors were regressed, developed an immune memory and rejected a challenge with an IL-1 non-producing violent tumor cell line. Endogenous IL-1α activates a cytokine cascade (i.e.; IL-6, CSF), produced by the malignant cells and possibly also by stromal cells. However, IL-1α expression is essential for fibrosarcoma eradication, while other cytokines possibly amplify and sustain its action. IL-1 non-producing fibrosarcomas can be induced to express IL-1α in a transient manner, by treatment in culture with cytokine/LPS, and subsequently they shift from progressor to regressor tumors. Positive immunotherapeutical effects were obtained when treating mice bearing IL-1 non-producing fibrosarcomas with cells from the same line, induced to express IL-1α in a constitutive or transient manner. Thus, IL-1α expression by malignant cells bears the potential to be applied in immunotherapy, as it is assumed that most naturally occurring tumors do not express IL-1α activity. Further studies are aimed at the elucidation of the mechanisms of IL-1α-mediated tumor regression and on the development of immunotherapy protocols based on its expression by malignant cells. |
| Databáze: | OpenAIRE |
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