Electroacupuncture promotes the survival and synaptic plasticity of hippocampal neurons and improvement of sleep deprivation‐induced spatial memory impairment
| Autor: | Zhi-Ling Li, Xin Zhou, Boyu Jiao, Hao-Yu Xu, Qing-Wen Deng, Fanqi Meng, Ying Ding, Jiuqing Tan, Yuan Gu, Wenya Pei, Jing-Wen Ruan, Guan-heng He, Baobao Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
MAPK/ERK pathway
Male Cell Survival memory impairment Morris water navigation task Tropomyosin receptor kinase B Biology Hippocampal formation Hippocampus Rats Sprague-Dawley Neurotrophic factors Physiology (medical) Animals Pharmacology (medical) Cells Cultured Spatial Memory Pharmacology Brain-derived neurotrophic factor Neurons brain‐derived neurotrophic factor Memory Disorders synaptic plasticity Neuronal Plasticity Behavior Animal Brain-Derived Neurotrophic Factor Neurogenesis Original Articles sleep deprivation Rats Psychiatry and Mental health Electroacupuncture nervous system hippocampal neuron Synaptic plasticity Original Article Neuroscience |
| Zdroj: | CNS Neuroscience & Therapeutics |
| ISSN: | 1755-5949 1755-5930 |
| Popis: | Aims This study aimed to investigate whether electroacupuncture (EA) promotes the survival and synaptic plasticity of hippocampal neurons by activating brain‐derived neurotrophic factor (BDNF)/tyrosine receptor kinase (TrkB)/extracellular signal‐regulated kinase (Erk) signaling, thereby improving spatial memory deficits in rats under SD. Methods In vivo, Morris water maze (MWM) was used to detect the effect of EA on learning and memory, at the same time Western blotting (WB), immunofluorescence (IF), and transmission electron microscopy (TEM) were used to explore the plasticity of hippocampal neurons and synapses, and the expression of BDNF/TrkB/Erk signaling. In vitro, cultured hippocampal neurons were treated with exogenous BDNF and the TrkB inhibitor K252a to confirm the relationship between BDNF/TrkB/Erk signaling and synaptic plasticity. Results Our results showed that EA mitigated the loss of hippocampal neurons and synapses, stimulated hippocampal neurogenesis, and improved learning and memory of rats under SD accompanied by upregulation of BDNF and increased phosphorylation of TrkB and Erk. In cultured hippocampal neurons, exogenous BDNF enhanced the expression of synaptic proteins, the frequency of the postsynaptic currents, and the phosphorylation of TrkB and Erk; these effects were reversed by treatment with K252a. Conclusions Electroacupuncture alleviates SD‐induced spatial memory impairment by promoting hippocampal neurogenesis and synaptic plasticity via activation of BDNF/TrkB/Erk signaling, which provided evidence for EA as a therapeutic strategy for countering the adverse effects of SD on cognition. Electroacupuncture may ameliorate spatial memory impairment during sleep deprivation through improving hippocampal neuron survival, neurogenesis and synaptic plasticity via regulating BDNF/TrkB/ERK signaling |
| Databáze: | OpenAIRE |
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