YKL-40 secreted from adipose tissue inhibits degradation of type I collagen
| Autor: | Akiko Sukeno, Masamichi Kuwajima, Takeo Iwata, Martin Wabitsch, Noriko Mizusawa, Yoshio Hayashi, Mitsuo Itakura, Naozumi Ishimaru, Katsuhiko Yoshimoto |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male musculoskeletal diseases medicine.medical_specialty YKL-40 preadipocyte Adipose tissue macrophages Biophysics Adipose tissue Inflammation macrophage Biochemistry Collagen Type I Extracellular matrix Adipokines Lectins Internal medicine Adipocytes medicine Humans Macrophage Chitinase-3-Like Protein 1 Obesity Molecular Biology Cells Cultured Aged Glycoproteins MMP-1 Chemistry Macrophages Cell Biology Middle Aged Stromal vascular fraction type I collagen Coculture Techniques Cell biology Collagen type I alpha 1 Endocrinology Adipose Tissue Female Matrix Metalloproteinase 1 Stromal Cells medicine.symptom Type I collagen |
| Zdroj: | Biochemical and Biophysical Research Communications. 388:511-516 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2009.08.024 |
| Popis: | Obesity is considered a chronic low-grade inflammatory status and the stromal vascular fraction (SVF) cells of adipose tissue (AT) are considered a source of inflammation-related molecules. We identified YKL-40 as a major protein secreted from SVF cells in human visceral AT. YKL-40 expression levels in SVF cells from visceral AT were higher than in those from subcutaneous AT. Immunofluorescence staining revealed that YKL-40 was exclusively expressed in macrophages among SVF cells. YKL-40 purified from SVF cells inhibited the degradation of type I collagen, a major extracellular matrix of AT, by matrix metalloproteinase (MMP)-1 and increased rate of fibril formation of type I collagen. The expression of MMP-1 in preadipocytes and macrophages was enhanced by interaction between these cells. These results suggest that macrophage/preadipocyte interaction enhances degradation of type I collagen in AT, meanwhile, YKL-40 secreted from macrophages infiltrating into AT inhibits the type I collagen degradation. |
| Databáze: | OpenAIRE |
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