Differential immunohistochemical localization of cytokeratins and collagen types I and III in experimentally-induced cirrhosis
| Autor: | M. S. Al Adnani |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
Pathology medicine.medical_specialty Cirrhosis Bile Duct Epithelium Connective tissue Biology Liver Cirrhosis Experimental Pathology and Forensic Medicine Immunoenzyme Techniques Collagen Type III Cytokeratin Keratin medicine Animals chemistry.chemical_classification Immunoperoxidase Rats Inbred Strains medicine.disease Rats medicine.anatomical_structure Liver chemistry Hepatocyte Keratins Collagen |
| Zdroj: | The Journal of Pathology. 159:151-158 |
| ISSN: | 1096-9896 0022-3417 |
| DOI: | 10.1002/path.1711590210 |
| Popis: | Liver cirrhosis was induced in male Wistar rats by subcutaneous injection (1 ml of 30 g/l) of an aqueous solution of thioacetamide. Using the indirect immunoperoxidase technique, high molecular weight keratins were localized in bile ducts and ductules. Low molecular weight cytokeratins were present in regenerating hepatocytes in active cirrhosis; bile ducts were unstained. These results suggest that cytokeratin staining may be useful in distinguishing bile duct epithelium and hepatocytes in hepatobiliary diseases. Anticollagen type III antibody stained hepatocytes and thin connective tissue fibres, while anticollagen type I antibody stained thicker fibres and some sinusoidal cells but not hepatocytes. Collagens were usually undetectable in normal liver cells. It is suggested, therefore, that hepatocytes may play a major role in collagen type III production which precedes the deposition of collagen type I. By contrast, collagen type I may be produced by fibroblasts and some cells along sinusoids (e.g. perisinusoidal fat-storing cells) after liver injury. |
| Databáze: | OpenAIRE |
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