Flaviaesturariibacter luteus sp. nov., isolated from an agricultural floodplain soil, and emended description of the genus Flaviaesturariibacter
| Autor: | Katharina J. Huber, Bärbel U. Foesel, Alicia Geppert, Javier Pascual, Jörg Overmann |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Bacterial Biology Microbiology 03 medical and health sciences Glycolipid Genus RNA Ribosomal 16S Botany Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Soil Microbiology chemistry.chemical_classification Base Composition Strain (chemistry) Bacteroidetes Fatty Acids Nucleic Acid Hybridization Agriculture Vitamin K 2 General Medicine Metabolism Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Namibia Amino acid Bacterial Typing Techniques 030104 developmental biology chemistry Soil microbiology |
| Zdroj: | International journal of systematic and evolutionary microbiology. 67(6) |
| ISSN: | 1466-5034 |
| Popis: | A novel slow-growing bacterial strain designated as AW305T was isolated from an agricultural floodplain soil located in Mashare, Kavango region, Namibia. Cells stained Gram-negative, were non-motile, non-spore-forming, coccoid to rod-shaped and did not form a capsule. Colonies were yellow-pigmented, but flexirubin-type pigments were not detected. AW305T had an aerobic chemo-organoheterotrophic metabolism, using a narrow spectrum of carbon sources for growth, with preference for complex protein substrates, organic acids and amino acids. AW305T was able to grow at 15–40 °C, pH 5.3–8.3 and in the presence of up to 0.25 % (w/v) NaCl. 16S rRNA gene sequence comparison showed that AW305T belonged to the genus Flaviaesturariibacter (family Chitinophagaceae ). Its closest relatives were Flaviaesturariibacter amylovorans GCR0105T (97.0 %), Flavisolibacter ginsengiterrae Gsoil 492T (93.6 %) and Flavisolibacter ginsengisoli Gsoil 643T (93.2 %). DNA–DNA hybridization experiments corroborated that AW305T represents an independent genomospecies. The genomic DNA G+C content was 57.6 mol%. Major fatty acids were iso-C15 : 1 G, iso-C15 : 0, C16 : 1ω5c, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The predominant respiratory quinone was MK-7, albeit minor amounts of MK-6 were also detected. The polar lipids comprised major amounts of phosphatidylethanolamine and minor amounts of two unidentified lipids, an unidentified phospholipid, an unidentified glycolipid and an unidentified aminoglycophospholipid. On the basis of the polyphasic characterization, strain AW305T represents a novel species of the genus Flaviaesturariibacter for which the name Flaviaesturariibacter luteus sp. nov. is proposed, with the type strain AW305T (=DSM 100282T=LMG 29416T). |
| Databáze: | OpenAIRE |
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