Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)
| Autor: | Kalenahalli Yogendra, Hemlatha Sanivarapu, Kalyani Prasad, Shiva Bhargavi Lingampali, Kiran K. Sharma, Pooja Bhatnagar-Mathur, Santisree Parankusam |
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| Rok vydání: | 2021 |
| Předmět: |
Aflatoxin
Arachis Aspergillus flavus Plant Science Biology Proteomics SB1-1110 Microbiology Human health proteomics aspergillus flavus parasitic diseases heterocyclic compounds QK900-989 Plant ecology skin and connective tissue diseases Ecology Evolution Behavior and Systematics business.industry aflatoxin Plant culture food and beverages Fungal pathogen biology.organism_classification Food safety Arachis hypogaea arachis hypogaea flavonoids peanut business phenylpropanoids |
| Zdroj: | Journal of Plant Interactions, Vol 16, Iss 1, Pp 494-509 (2021) |
| ISSN: | 1742-9153 1742-9145 |
| Popis: | Aspergillus flavus is an opportunistic fungal pathogen that produces carcinogenic aflatoxin, a serious constraint for food safety and human health. In this study, to better understand the molecular mechanism/s of peanut resistance to A. flavus growth and aflatoxin accumulation, comparative proteomic analysis was performed in two contrasting peanut genotypes, variety JL 24 (susceptible) and its near-isogenic resistant transgenic derivative expressing an alfalfa defensin gene. Several resistance proteins associated with secondary metabolic pathways were strongly induced in the resistant genotypes including phenylalanine ammonia lyase, cinnamic acid-4-hydroxylase, chalcone synthase, resveratrol synthase, flavanone-3-hydroxylase, lipoxygenase, diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase, β-ketoacyl-ACP-reductase, monoacylglycerol acyltransferase, and diacylglycerol acyltransferase, indicating their roles in resistance. Besides, several putative susceptibility-associated proteins were revealed providing knowledge on potential candidate target genes for precise breeding interventions for aflatoxin mitigation. This is the first study to demonstrate comparative proteomics analysis in Aspergillus–peanut interaction using contrasting near-isogenic lines to elucidate the underlying molecular mechanisms of resistance. |
| Databáze: | OpenAIRE |
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