Purification, crystallization and preliminary X-ray crystallographic studies of swine MHC class I complexed with an FMDV CTL epitope Hu64
| Autor: | Lei Feng, Feng-shan Gao, Zi-bin Li, Hua Gao, Zong-hui Zhang, Yong Han, Ming-wei Sun, Ping Jiang, Xiao-xin Zhai |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Swine viruses animal diseases Epitopes T-Lymphocyte Context (language use) Major histocompatibility complex Epitope Virus 03 medical and health sciences MHC class I Cytotoxic T cell Animals General Veterinary biology Chemistry X-Rays fungi Haplotype Histocompatibility Antigens Class I Virology CTL 030104 developmental biology Foot-and-Mouth Disease Virus biology.protein Crystallization |
| Zdroj: | Research in veterinary science. 119 |
| ISSN: | 1532-2661 |
| Popis: | Up to now, no crystal structure of swine leukocyte antigen 2 (SLA-2) molecules was reported. In order to elucidate the structure of SLA-2 and to study the cytotoxic T lymphocyte (CTL) epitopes derived from foot-and-mouth disease virus (FMDV), a complex of swine major histocompatibility complex (MHC) class I molecule (SLA-2 haplotype, Hebao allele) with swine β2-microglobulin and the CTL epitope FMDV-Hu64 (ALLRTATYY) derived from O serotype of FMDV VP1 protein (residues 64-72) was refolded and crystallized. The crystal, which belonged to space group P212121, diffracted to 2.5 A resolution and had unit cell parameters a = 48.37, b = 97.75, c = 166.163 A. These results will help to determine the first structure of a SLA-2 molecule in the context of an FMDV CTL epitope. |
| Databáze: | OpenAIRE |
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