A291 INHIBITORY EFFECTS OF GHRELIN ON MEMBRANE EXCITABILITY AND RESPONSES TO SATIETY MEDIATORS IN VAGAL AFFERENT NEURONS: INVOLVEMENT OF SUPPRESSOR OF CYTOKINE SIGNALING 3
| Autor: | S Park, M J Beyak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Chemistry digestive oral and skin physiology Nodose Ganglion medicine.disease Inhibitory postsynaptic potential Paper Sessions Endocrinology medicine.artery Internal medicine Pulmonary artery medicine Polyarthritis Ghrelin Serotonin SOCS3 Plasminogen activator hormones hormone substitutes and hormone antagonists |
| Popis: | BACKGROUND: The orexigenic hormone ghrelin regulates feeding behavior via vagal afferent pathways. The mechanisms by which ghrelin exerts its inhibitory effects on nodose ganglion neurons are largely unknown. There is a evidence that ghrelin caused a significant increase in suppressor of cytokine signaling 3 (SOCS3) expression in nodose ganglion neurons, the cell bodies of vagal afferents. AIMS: The aim of this study was to examine the effects of ghrelin on excitability and satiety mediator responses and whether SOCS3 is associated with the effects of ghrelin on nodose neurons. METHODS: Nodose neurons from C57Bl6J mice were dissociated and incubated overnight with ghrelin (100 nM) and with/without zoledronic acid (ZA, a SOCS3 inhibitor, 10 mM). Current clamp recordings (to examine excitability) and Fura-2AM Ca(2+)-imaging (to examine responses to satiety mediators) were performed 18–24 h post-dissociation. RESULTS: Ghrelin (100 nM) significantly decreased the membrane potential (-52.0 ± 0.9 mV (n=18, control) vs. -61.5 ± 1.9 mV (n=15, ghrelin), unpaired student t-test, p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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