Modulation of soluble phases of endothelial/leukocyte adhesion molecule 1, intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 with interleukin-1beta after experimental endotoxic challenge
| Autor: | José Antonio Ferrón Orihuela, Luis Téllez Gil, Rafael Lupiani Moreno, Alfonso Mansilla Roselló, Antonia Collado Torres |
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| Rok vydání: | 2001 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Ratón medicine.medical_treatment Intercellular Adhesion Molecule-1 Vascular Cell Adhesion Molecule-1 Inflammation Pharmacology Critical Care and Intensive Care Medicine chemistry.chemical_compound Mice Medicine Animals Saline Escherichia coli Infections Analysis of Variance business.industry Cell adhesion molecule Biological activity Cytokine chemistry Immunology Mice Inbred CBA Female medicine.symptom business E-Selectin Interleukin-1 |
| Zdroj: | Critical care medicine. 29(4) |
| ISSN: | 0090-3493 |
| Popis: | OBJECTIVE To evaluate the effect of treatment with interleukin 1beta (IL-1beta) on the concentrations of soluble adhesion molecules after an endotoxic challenge. DESIGN Randomized, controlled study. SETTING Experimental Unit, Virgen de las Nieves University Hospital. SUBJECTS Seventy-two female CBA/H mice of 20 to 21 g, supplied by the animal center of the Experimental Unit. INTERVENTION The mice were randomized into three groups of 24. Group 1 (sham) received two intraperitoneal (ip) doses of 0.1 mL of phosphate-buffered saline; group 2 (lipopolysaccharide) was injected with 125 mg/kg lipopolysaccharide (Escherichia coli) (i.p.) 24 hrs after 0.1 mL of phosphate-buffered saline; group 3 was pretreated with 80 ng (i.p.) of IL-1beta per mouse 24 hrs before the endotoxic challenge. MEASUREMENTS AND MAIN RESULTS At 1, 2, 4, and 24 hrs after the endotoxic challenge, the concentrations of soluble endothelial/leukocyte adhesion molecule 1 (ELAM-1), intercellular adhesion molecule 1 (ICAM-1), and vascular cell adhesion molecule 1 (VCAM-1) were measured in the three groups. There was a significant increase (p |
| Databáze: | OpenAIRE |
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