Ion trap detector — capillary gas chromatography of valproic acid and its mono-unsaturated metabolites in serum using methyl ester derivatives
| Autor: | Vitto M, Laureri Cf, Enrico Gaetani |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Chromatography
Calibration curve Chemistry Capillary action Valproic Acid Clinical Biochemistry Pharmaceutical Science Stereoisomerism Mass spectrometry Methylation Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Calibration Drug Discovery Humans Selected ion monitoring Ion trap Gas chromatography Gas chromatography–mass spectrometry Derivatization Spectroscopy |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 10:193-197 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/0731-7085(92)80028-l |
| Popis: | A quantitative method was developed for valproic acid and five of its mono-unsaturated metabolites using capillary gas chromatography—mass spectrometry with selected ion monitoring. The method was applied to serum and all metabolites were measured in a single run. Methyl esters were synthesized as the derivatives suitable for gas chromatography. Calibration curves were found to be linear and the sensitivities in the order of 0.1 μg ml−1. Patients' data are presented. By this method it is possible to separate the stereoisomers of 2-n-propyl-2-pentenoic acid and of 2-n-propyl-3-pentenoic acid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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