Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
| Autor: | Richard G. J. Dohmen, Sophie Hubalek, Johanna Melke, Tobias Messmer, Federica Cantoni, Arianna Mei, Rui Hueber, Rada Mitic, Dirk Remmers, Panagiota Moutsatsou, Mark J. Post, Laura Jackisch, Joshua E. Flack |
|---|---|
| Přispěvatelé: | Fysiologie, RS: Carim - V03 Regenerative and reconstructive medicine vascular disease |
| Rok vydání: | 2021 |
| Předmět: |
EXPRESSION
Cell biology MEAT Nutrition. Foods and food supply FATTY-ACID PROFILES REGULATORY CHALLENGES Public Health Environmental and Occupational Health food and beverages Agriculture TP368-456 Food processing and manufacture Article FIBRO/ADIPOGENIC PROGENITORS PALATABILITY DIFFERENTIATION Mesenchymal stem cells TX341-641 BEEF STEM-CELLS Food Science SUPPRESSION |
| Zdroj: | NPJ Science of Food npj Science of Food, Vol 6, Iss 1, Pp 1-12 (2022) npj Science of Food, 6(1):6. Springer Nature |
| ISSN: | 2396-8370 |
| Popis: | Cultured meat is an emergent technology with the potential for significant environmental and animal welfare benefits. Accurate mimicry of traditional meat requires fat tissue; a key contributor to both the flavour and texture of meat. Here, we show that fibro-adipogenic progenitor cells (FAPs) are present in bovine muscle, and are transcriptionally and immunophenotypically distinct from satellite cells. These two cell types can be purified from a single muscle sample using a simple fluorescence-activated cell sorting (FACS) strategy. FAPs demonstrate high levels of adipogenic potential, as measured by gene expression changes and lipid accumulation, and can be proliferated for a large number of population doublings, demonstrating their suitability for a scalable cultured meat production process. Crucially, FAPs reach a mature level of adipogenic differentiation in three-dimensional, edible hydrogels. The resultant tissue accurately mimics traditional beef fat in terms of lipid profile and taste, and FAPs thus represent a promising candidate cell type for the production of cultured fat. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|