The 5.8S pre‐rRNA maturation factor, M‐phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis
| Autor: | Wen-Tao Zeng, Wen-Yi Gao, Fan Hu, Zhi-Xia Yang, Dong Zhang, Jing-Yang Cai, Feng-Yu Zhu, Rui-Rui Peng, Xi-Chen Chen, Zheng-Rong Xia, Li-Ya Shi, Li-Li Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Somatic cell Cell Cycle Proteins Fertilization in Vitro Biology female fertility factor 03 medical and health sciences Mice 0302 clinical medicine Meiosis Western blot medicine Animals oocyte Microinjection Cells Cultured Cellular Senescence Mice Inbred ICR medicine.diagnostic_test RNA-Binding Proteins Cell Biology General Medicine Original Articles Ribosomal RNA Oocyte Cell biology RNA Ribosomal 5.8S 5.8S Pre‐rRNA 030104 developmental biology medicine.anatomical_structure Fertility quality 030220 oncology & carcinogenesis Phosphoprotein M‐phase phosphoprotein 6 Oocytes Original Article Female Cell Division Fluorescence in situ hybridization |
| Zdroj: | Cell Proliferation |
| ISSN: | 1365-2184 0960-7722 |
| Popis: | Objectives M‐phase phosphoprotein 6 (MPP6) is important for 5.8S pre‐rRNA maturation in somatic cells and was screened as a female fertility factor. However, whether MPP6 functions in oocyte meiosis and fertility is not yet known. We aimed to address this. Materials and Methods Mouse oocytes with surrounded nucleus (SN) or non‐surrounded nucleus (NSN) were used for all experiments. Peptide nanoparticle‐mediated antibody transfection was used to deplete MPP6. Immunofluorescence staining, immunohistochemistry and live tracker staining were used to examine MPP6 localization and characterize phenotypes after control or MPP6 depletion. High‐fidelity PCR and fluorescence in situ hybridization (FISH) were used to examine the localization and level of 5.8S rRNAs. Western blot was used to examine the protein level. MPP6‐EGFP mRNA microinjection was used to do the rescue. Results MPP6 was enriched within ovaries and oocytes. MPP6 depletion significantly impeded oocyte meiosis. MPP6 depletion increased 5.8S pre‐rRNA. The mRNA levels of MPP6 and 5.8S rRNA decreased within ageing oocytes, and MPP6 mRNA injection partially increased 5.8S rRNA maturation and improved oocyte quality. Conclusions MPP6 is required for 5.8S rRNA maturation, meiosis and quality control in mouse oocytes, and MPP6 level might be a marker for oocyte quality. |
| Databáze: | OpenAIRE |
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