Altered acetylcholine, bradykinin and cutaneous pressure‐induced vasodilation in mice lacking the TREK1 potassium channel: the endothelial link

Autor: Frédéric Brau, Michel Lazdunski, Ambroise Garry, Nicolas Blondeau, Bérengère Fromy, Daniel Henrion, Jean Louis Saumet, Catherine Heurteaux, Nicolas Guy, Pierre Gounon
Přispěvatelé: Biologie Neurovasculaire Intégrée (BNVI), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Centre commun de microscopie appliquée, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Loudig, Nadine, Université Nice Sophia Antipolis (... - 2019) (UNS)
Rok vydání: 2007
Předmět:
MESH: Mice
Mutant Strains
MESH: Mesenteric Arteries
Blood Pressure
Vasodilation
Biochemistry
Mice
chemistry.chemical_compound
two-pore-domain K þ channel
0302 clinical medicine
MESH: Animals
Endothelial dysfunction
Mesenteric arteries
0303 health sciences
Chemistry
MESH: Potassium Channels
Tandem Pore Domain
MESH: Blood Pressure
Potassium channel
Mesenteric Arteries
[SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
medicine.anatomical_structure
MESH: Endothelium
Vascular
MESH: Pressure
Acetylcholine
medicine.drug
skin
medicine.medical_specialty
Intracellular pH
Scientific Report
microcirculation
Bradykinin
Nitric Oxide
Nitric oxide
MESH: Vasodilation
03 medical and health sciences
Potassium Channels
Tandem Pore Domain
MESH: Skin
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Internal medicine
Pressure
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
Genetics
medicine
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
MESH: Mice
Molecular Biology
030304 developmental biology
MESH: Capillaries
MESH: Bradykinin
MESH: Acetylcholine
medicine.disease
Mice
Mutant Strains
Capillaries
Endocrinology
MESH: Gene Deletion
MESH: Nitric Oxide
Endothelium
Vascular
Gene Deletion
030217 neurology & neurosurgery
Zdroj: EMBO Reports
EMBO Reports, 2007, 8 (4), pp.354-9. ⟨10.1038/sj.embor.7400916⟩
EMBO Reports, EMBO Press, 2007, 8 (4), pp.354-9. ⟨10.1038/sj.embor.7400916⟩
ISSN: 1469-3178
1469-221X
DOI: 10.1038/sj.embor.7400916
Popis: The TWIK related K+ channel TREK1 is an important member of the class of two-pore-domain K+ channels. It is a background K+ channel and is regulated by hormones, neurotransmitters, intracellular pH and mechanical stretch. This work shows that TREK1 is present both in mesenteric resistance arteries and in skin microvessels. It is particularly well expressed in endothelial cells. Deletion of TREK1 in mice leads to an important alteration in vasodilation of mesenteric arteries induced by acetylcholine and bradykinin. Iontophoretic delivery of acetylcholine and bradykinin in the skin of TREK1+/+ and TREK1−/− mice also shows the important role of TREK1 in cutaneous endothelium-dependent vasodilation. The vasodilator response to local pressure application is also markedly decreased in TREK1−/− mice, mimicking the decreased response to pressure observed in diabetes. Deletion of TREK1 is associated with a marked alteration in the efficacy of the G-protein-coupled receptor-associated cascade producing NO that leads to major endothelial dysfunction.
Databáze: OpenAIRE
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