Neuroprotection of catalpol in transient global ischemia in gerbils
| Autor: | Ying Liu, Li Jia An, Yan Long Duan, Dan Qing Li, Yong Ming Bao, Chui Ping Liu |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Lipid Peroxides Iridoid Glucosides Apoptosis Pharmacology Hippocampus Neuroprotection Brain Ischemia Nitric oxide chemistry.chemical_compound Glucosides In Situ Nick-End Labeling Animals Hippocampus (mythology) Iridoids Maze Learning Neurons chemistry.chemical_classification Glutathione Peroxidase Behavior Animal biology Lipid peroxide General Neuroscience Glutathione peroxidase General Medicine Catalpol Nitric oxide synthase Disease Models Animal Neuroprotective Agents chemistry Biochemistry biology.protein Female Nitric Oxide Synthase Gerbillinae |
| Zdroj: | Neuroscience Research. 50:169-177 |
| ISSN: | 0168-0102 |
| DOI: | 10.1016/j.neures.2004.06.009 |
| Popis: | The neuroprotection of catalpol and its mechanism was evaluated in cerebral ischemic model in gerbils. Three groups were designed as sham-operated, ischemia-treated, respectively, with catalpol and saline. Catalpol was injected intraperitoneally immediately after reperfusion and repeatedly at 12, 24, 48 and 72 h with the dose of 5.0 mg/kg. The neuroprotection was estimated by the indexes of behavior and histology. Behavioral testing was performed in Y-maze and the survival neurons in CA1 subfield were counted under a microscope after behavioral testing. In addition, apoptosis induced by ischemia was also examined by using the terminal deoxynucleotidyl transferase-mediated UTP nick end labeling method. It was shown that catalpol significantly attenuated apoptosis, rescued hippocampal CA1 neurons and reduced cognitive impairment. In order to make clear the mechanism of catalpol’s neuroprotection, the activities of endogenous antioxidants and nitric oxide synthase together with the content of lipid peroxide in cortex and hippocampus were assayed. The results proved that catalpol significantly reduced the content of lipid peroxide, increased the activity of glutathione peroxidase and decreased the activity of nitric oxide synthase. All these suggested that catalpol was a potential neuroprotective agent and its neuroprotective effects were achieved at least partly by promoting endogenous antioxidant enzymatic activities and reducing the formation of nitric oxide. |
| Databáze: | OpenAIRE |
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