NF-kappaB Regulates Redox Status in Breast Cancer Subtypes
| Autor: | Rubens Cecchini, Carolina Panis, Gerson M. Ferreira, Bruno R. B. Pires, Eliana Abdelhay, Renata Binato |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genome instability Microarray lcsh:QH426-470 Context (language use) Biology medicine.disease_cause Article 03 medical and health sciences 0302 clinical medicine breast cancer Gene expression Genetics medicine oxidative stress Transcription factor Genetics (clinical) Gene knockdown subtypes lcsh:Genetics 030104 developmental biology 030220 oncology & carcinogenesis redox Cancer cell Cancer research gene expression NFkappaB microarray Oxidative stress |
| Zdroj: | Genes Volume 9 Issue 7 Genes, Vol 9, Iss 7, p 320 (2018) |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes9070320 |
| Popis: | Oxidative stress (OS) is an indispensable condition to ensure genomic instability in cancer cells. In breast cancer (BC), redox alterations have been widely characterized, but since this process results from a chain of inflammatory events, the causal molecular triggers remain to be identified. In this context, we used a microarray approach to investigate the role of the main pro-oxidant transcription factor, nuclear factor-kappa B (NF-κB), in gene profiles of BC subtypes. Our results showed that NF-κB knockdown in distinct BC subtypes led to differential expression of relevant factors involved in glutathione metabolism, prostaglandins, cytochrome P450 and cyclooxygenase, suggesting a relationship between the redox balance and NF-κB in such cells. In addition, we performed biochemical analyses to validate the microarray dataset focusing on OS and correlated these parameters with normal expression or NF-κB inhibition. Our data showed a distinct oxidative status pattern for each of the three studied BC subtype models, consistent with the intrinsic characteristics of each BC subtype. Thus, our findings suggest that NF-κB may represent an additional mechanism related to OS maintenance in BC, operating in various forms to mediate other important predominant signaling components of each BC subtype. |
| Databáze: | OpenAIRE |
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