Lyophilized Silk Sponges: A Versatile Biomaterial Platform for Soft Tissue Engineering
| Autor: | Kelly A. Burke, Jelena Rnjak-Kovacina, Wenwen Huang, Julianne M. Golinski, Lindsay Wray, Tess Torregrosa, David L. Kaplan |
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| Rok vydání: | 2015 |
| Předmět: |
Scaffold
Materials science Biomedical Engineering lyophilized sponge macromolecular substances 02 engineering and technology scaffold 010402 general chemistry 01 natural sciences Article Biomaterials Crystallinity Soft tissue engineering silk Regeneration (biology) fungi Mesenchymal stem cell biomaterial technology industry and agriculture Biomaterial Adhesion equipment and supplies 021001 nanoscience & nanotechnology 0104 chemical sciences SILK soft tissue engineering Chemical engineering 0210 nano-technology Biomedical engineering |
| Zdroj: | ACS Biomaterials Science & Engineering |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/ab500149p |
| Popis: | We present a silk biomaterial platform with highly tunable mechanical and degradation properties for engineering and regeneration of soft tissues such as, skin, adipose, and neural tissue, with elasticity properties in the kilopascal range. Lyophilized silk sponges were prepared under different process conditions and the effect of silk molecular weight, concentration and crystallinity on 3D scaffold formation, structural integrity, morphology, mechanical and degradation properties, and cell interactions in vitro and in vivo were studied. Tuning the molecular weight distribution (via degumming time) of silk allowed the formation of stable, highly porous, 3D scaffolds that held form with silk concentrations as low as 0.5% wt/v. Mechanical properties were a function of silk concentration and scaffold degradation was driven by beta-sheet content. Lyophilized silk sponges supported the adhesion of mesenchymal stem cells throughout 3D scaffolds, cell proliferation in vitro, and cell infiltration and scaffold remodeling when implanted subcutaneously in vivo. |
| Databáze: | OpenAIRE |
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