Centrifugal enhancement of human immunodeficiency virus (HIV) and human herpesvirus type 6 (HHV-6) infection in vitro
| Autor: | Gerald B. Harnett, Marion R. Bucens, Glenys R. Pietroboni |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Infectivity
biology viruses Fluorescent Antibody Technique HIV virus diseases Centrifugation Transfection In Vitro Techniques Virus Replication biology.organism_classification Virology Virus In vitro Cell Line Microbiology Viral replication Cell culture Peripheral blood lymphocyte Humans Human herpesvirus 6 Lymphocytes Herpesviridae |
| Zdroj: | Journal of Virological Methods. 24:85-90 |
| ISSN: | 0166-0934 |
| Popis: | The effect of centrifugal inoculation of human immunodeficiency virus (HIV) and human herpesvirus-6 (HHV-6) on the infectivity of the viruses for cell cultures was examined. Three HIV-1 strains, ARV-2, HTLV-IIIb and a local isolate, WA-46c, were tested in peripheral blood lymphocytes, HUT-78, H9 and MT-2 cells. The HHV-6 strain was a local isolate and was studied only in peripheral blood lymphocyte cultures. Centrifugal inoculation of the viruses at a force of 2500 x g for 60 min, enhanced HIV-1 infectivity by a factor of about 10-fold in all cell cultures tested. Infectivity was increased about 100-fold for HHV-6. |
| Databáze: | OpenAIRE |
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