Sulforaphane protects brains against hypoxic–ischemic injury through induction of Nrf2-dependent phase 2 enzyme
| Autor: | Ni Cheng, Jianmei Cai, Qiusha Wang, Shizhong Wang, Zhang Ping, John H. Zhang, Xuejun Sun, Sujian Wang, Zhimin Kang, Wenwu Liu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty NF-E2-Related Factor 2 Apoptosis Pharmacology medicine.disease_cause Second Messenger Systems Neuroprotection Rats Sprague-Dawley Lipid peroxidation chemistry.chemical_compound Isothiocyanates NAD(P)H Dehydrogenase (Quinone) medicine Animals Molecular Biology chemistry.chemical_classification Reactive oxygen species TUNEL assay General Neuroscience Malondialdehyde Rats Heme oxygenase Disease Models Animal Neuroprotective Agents Animals Newborn chemistry Enzyme Induction Sulfoxides Heme Oxygenase (Decyclizing) Hypoxia-Ischemia Brain Nerve Degeneration Neurology (clinical) Biomarkers Thiocyanates Oxidative stress Developmental Biology Sulforaphane |
| Zdroj: | Brain Research. 1343:178-185 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2010.04.036 |
| Popis: | Neonatal hypoxia-ischemia (HI) brain injury involves reactive oxygen species (ROS) and inflammatory responses. Sulforaphane (SFN), an isothiocyanate found in cruciferous vegetables, has cytoprotective effects against oxidative stress and its effect was mediated by NF-E2-related factor-2 (Nrf2), a transcription factor, and heme oxygenase 1 (HO-1) which is one of Nrf2 downstream target genes. This study was undertaken to investigate the neuroprotective mechanisms of SFN in a neonatal HI rat model. Seven-day-old rat pups were subjected to left common carotid artery ligation and hypoxia (8% oxygen at 37 degrees C) for 90 min. SFN (5mg/kg) was systemically administered 30 min before HI insult. Brain injury was assessed by 2,3,5-triphenyltetrazoliumchloride (TTC), Nissl, TUNEL staining, malondialdehyde (MDA), 8OH-dG level, and caspase-3 activity in the cortex and hippocampus. SFN pretreatment increased the expression of Nrf2 and HO-1 in the brain and reduced infarct ratio at 24h after HI. The number of TUNEL-positive neurons as well as activated macroglia and the amount of 8OH-dG, were markedly reduced after SFN treatment, accompanied by suppressed caspase-3 activity and reduced lipid peroxidation (MDA) level. These results demonstrated that SFN could exert neuroprotective effects through increasing Nrf2 and HO-1 expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect