Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene
| Autor: | Walter Ajambang, Culbertson Enow Etta, Ardha Apriyanto, Purnama Hidayat, Bandung Sahari, Van Basten Tambunan, Damayanti Buchori |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mitochondrial DNA QH301-705.5 Population Plant Science 010603 evolutionary biology 01 natural sciences DNA barcoding Haplogroup 03 medical and health sciences Monophyly Genetic variation parasitic diseases Biology (General) education Molecular Biology education.field_of_study biology Phylogenetic tree Weevil biology.organism_classification coi gene elaeidobius kamerunicus mtdna oil palm weevil 030104 developmental biology Evolutionary biology Animal Science and Zoology |
| Zdroj: | Biodiversitas, Vol 21, Iss 7 (2020) |
| ISSN: | 2085-4722 3263-3270 |
| Popis: | Tambunan VB, Apriyanto A, Ajambang W, Etta CE, Sahari B, Buchori D, Hidayat P. 2020. Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene. Biodiversitas 21: 3263-3270. Oil palm pollinating weevil Elaeidobius kamerunicus is a very important insect pollinator in oil palm plantation. However, there is still lack of information about molecular identification and population genetic study in this species. The purpose of this study was to explore the effectiveness of oil palm pollinating weevil identification using mitochondrial DNA of COI gene and to assess its genetic variation between different locations and countries. We sequenced the DNA barcode of 36 individuals of this species using the mtDNA Cytochrome Oxidase I (COI) gene to explore their genetic variation, identity and phylogenetic relationship. The COI gene sequences generated from this study were successful in identifying E. kamerunicus. Phylogenetic analysis also revealed 3 well-supported monophyletic haplogroups of E. kamerunicus population. In addition, genetic differentiation analysis revealed that most populations from Indonesia were different from Malaysian and Cameroonian populations indicating that there was a genetic variation between the population samples from these countries. The overall E. kamerunicus used in this study were geographically structured in two regions; outside Indonesia region (Cameroon and Malaysia) and Indonesia region. These results demonstrate the feasibility of using COI gene sequence for molecular identification and population genetic study of E. kamerunicus species. |
| Databáze: | OpenAIRE |
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