Study of modifying effects of astaxanthin on cytogenetic manifestations of bystander response in human peripheral blood lymphocytes in vitro
| Autor: | O.B. Dibska, O O Talan, M A Pilinska, V V Talko, O V Shemetun |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Chromosome Aberrations
Male Cancer Research Chronic lymphocytic leukemia Average level Radiation-Protective Agents Bystander Effect Xanthophylls medicine.disease Leukemia Lymphocytic Chronic B-Cell In vitro Peripheral blood chemistry.chemical_compound Oncology chemistry Astaxanthin Chromosome instability medicine Cancer research Bystander effect Humans Female Lymphocytes Cells Cultured |
| Zdroj: | Experimental oncology. 43(2) |
| ISSN: | 1812-9269 |
| Popis: | AIM To study the possible impact of astaxanthin on the cytogenetic manifestations of the simultaneous development of radiation-induced (RIBE) and tumor-induced bystander effect (TIBE) in human peripheral blood lymphocytes. MATERIALS AND METHODS Peripheral blood lymphocytes from healthy persons and patients with chronic lymphocytic leukemia were cultured separately or cocultured with or without previous irradiation in vitro by 137Cs at a dose of 0.5 Gy. The cells were cultured with and without 20 μg/ml astaxanthin. RESULTS In the presence of astaxanthin, the decrease of chromosomal instability both in the variant with separate TIBE and with simultaneous development of TIBE and RIBE was observed as the reduction in the frequency of simple chromosome-type aberrations, namely, double fragments. The average level of chromatid-type aberrations did not change under the action of astaxanthin. Although the total chromosome instability in bystander cells diminished, this did not lead to the elimination of the RIBE and TIBE development in the presence of astaxanthin. CONCLUSION In the setting of experiment, astaxanthin did not reduce the frequency of chromatid-type aberrations in bystander cells due to RIBE and TIBE but reduced the frequency of simple aberrations of chromosomal type, not associated with the development of bystander response phenomenon. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|