PD-L1 assessment in pediatric rhabdomyosarcoma: a pilot study
| Autor: | Patrizia Gasparini, Michela Casanova, Salvatore Lorenzo Renne, Stefano Chiaravalli, Luca Bergamaschi, Andrea Ferrari, Marta Barisella, Paola Collini, Massimo Milione, Cinzia Paolino, Cecilia Gardelli, Maura Massimino, Giulia Bertolini, Giovanni Centonze |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Cancer Research Pathology medicine.medical_specialty Pilot Projects Soft Tissue Neoplasms PD-L1 expression lcsh:RC254-282 B7-H1 Antigen Flow cytometry 03 medical and health sciences 0302 clinical medicine Immune system PD-L1 Rhabdomyosarcoma Biomarkers Tumor Genetics Humans Medicine Child Retrospective Studies Soft tissue sarcoma Primary cell lines biology medicine.diagnostic_test business.industry Immunohistrochemistry medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Immune checkpoint Pediatric malignancies 030104 developmental biology Oncology 030220 oncology & carcinogenesis biology.protein Immunohistochemistry Female Antibody business Research Article |
| Zdroj: | BMC Cancer, Vol 18, Iss 1, Pp 1-9 (2018) BMC Cancer |
| ISSN: | 1471-2407 |
| Popis: | Background Rhabdomyosarcomas (RMSs) are the most frequent soft tissue sarcoma in children and adolescents, defined by skeletal muscle differentiation and the status of FOXO1 fusions. In pediatric malignancies, in particular RMS, scant and controversial observations are reported about PD-L1 expression as a putative biomarker and few immune checkpoint clinical trials are conducted. Methods PD-L1 assessment was evaluated by immunohistochemistry (IHC) utilizing two anti-PDL1 antibodies, in a pilot cohort of 25 RMS. Results were confirmed in primary and commercial RMS cell lines by cytofluorimetric analysis and IHC. Results PD-L1 expression was detectable, by both anti-PD-L1 antibodies, in the immune contexture of immune cells infiltrating and/or surrounding the tumor, in 15/25 (60%) RMS, while absent expression was observed in neoplastic cells. Flow cytometry analysis and PD-L1 IHC of commercial and primary RMS cell lines confirmed a very small percentage of PD-L1 positive-tumor cells, under the detection limits of conventional IHC. Interestingly, increased PD-L1 expression was observed in the immune contexture of 4 RMS cases post chemotherapy compared to their matched pre-treatment samples. Conclusion Here we identify a peculiar pattern of PD-L1 expression in our RMS series with scanty positive-tumor cells detected by flow cytometry, and recurrent expression in the immune cells surrounding or infiltrating the tumor burden. Electronic supplementary material The online version of this article (10.1186/s12885-018-4554-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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