Reduced expression of vascular cell adhesion molecule-1 on bone marrow stromal cells isolated from marrow transplant recipients correlates with a reduced capacity to support human B lymphopoiesis in vitro
| Autor: | B N, Dittel, T W, LeBien |
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| Rok vydání: | 1995 |
| Předmět: |
B-Lymphocytes
Interleukin-7 Lymphoma Non-Hodgkin Immunology Vascular Cell Adhesion Molecule-1 Bone Marrow Cells Cell Differentiation Cell Biology Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma Flow Cytometry Hodgkin Disease Biochemistry Hematopoiesis Cell Adhesion Humans Interleukin-4 Stromal Cells Cell Adhesion Molecules Cell Division Cells Cultured Bone Marrow Transplantation Interleukin-1 |
| Zdroj: | Blood. 86:2833-2841 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | A common sequela to allogeneic or autologous bone marrow transplantation (BMT) is a delay in the reconstitution of a functional B-cell immune response. Therefore, we examined whether the posttransplant BM microenvironment is deficient in supporting the proliferation and/or differentiation of B-cell precursors. BM stromal cell cultures were established from patients who received allogeneic or autologous BMT for acute lymphoblastic leukemia, Hodgkin's disease, or non-Hodgkin's lymphoma. These cultures were then compared with normal donor BM stromal cell cultures for expression of adhesion molecules and the capacity to support the adhesion and interleukin-7 (IL-7)-dependent growth of normal B-cell precursors. Analysis of BM stromal cell cultures established from 28 BMT recipients showed a significantly reduced expression of cell surface vascular cell adhesion molecule-1 (VCAM-1/CD106), compared with normal donor BM stromal cells. Transplant BM stromal cell CD106 expression was responsive to regulatory cytokines in a manner qualitatively comparable with normal donor BM stromal cells. The level of B-cell precursor adhesion to transplant BM stromal cells correlated with the level of CD106 expression. Of 19 evaluable transplant BM stromal cell cultures, eight exhibited a reduced capacity to support the growth of CD19+/light chain-normal B-cell precursors. The capacity of transplant BM stromal cells to support B-cell precursor growth correlated with the level of CD106 expression, and the level of B-cell precursor adhesion. Our collective results may provide new mechanistic insight into why B-cell recovery is delayed post-BMT and underscore the importance of VCAM-1/CD106 in regulating B lymphopoiesis. |
| Databáze: | OpenAIRE |
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