Ratiometric enhanced fluorometric determination and imaging of intracellular microRNA-155 by using carbon dots, gold nanoparticles and rhodamine B for signal amplification
| Autor: | Baram Ahmed Hamah-Ameen, Abdollah Salimi, Fardin Fathi, Farzad Soleimani, Somayeh Hamd-Ghadareh |
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| Rok vydání: | 2018 |
| Předmět: |
Nanoprobe
Metal Nanoparticles 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Limit of Detection Cell Line Tumor Quantum Dots Rhodamine B Fluorescence Resonance Energy Transfer Humans Detection limit Rhodamines Nucleic Acid Hybridization RNA Probes 021001 nanoscience & nanotechnology Fluorescence Carbon 0104 chemical sciences MicroRNAs Förster resonance energy transfer chemistry Colloidal gold Biophysics Gold 0210 nano-technology Selectivity Conjugate |
| Zdroj: | Mikrochimica acta. 186(7) |
| ISSN: | 1436-5073 |
| Popis: | An ultrasensitive and highly reliable ratiometric assay is described for the determination of microRNA-155. It works at the attomolar concentration level and has high selectivity which warrants its potential application in cancer biomarker tracking. The excellent performance of this method results from (a) the use of a hybrid conjugate prepared from Rhodamine B (RhB), carbon dots (CDs) and probe-microRNA, and (b) from the measurement of fluorescence resonance energy transfer (FRET) that is observed in the AuNP/target-microRNA system as a result of RNA hybridization. The dye RhB (emission peak at 580 nm) serves as an internal reference. The sensitivity of this assay is increased by about 30% because of the broad emissions of CDs (489 nm and 665 nm) through a sequential FRET phenomenon. RhB-CDs were covalently bio-conjugated to probe microRNA. In the presence of AuNPs, the fluorescence of the CDs is quenched, while in the presence of microRNA-155, the ratio of fluorescences at 489 and 665 nm (I489/I665) is enhanced again. A linear relationship exists between the ratio of fluorescence and the concentration of microRNA-155 in the range from 1 aM to 0.1 μM, and the detection limit is 0.3 aM. The assay was applied to quantitative studies of target microRNA-155 in multiple pathways associated with cancer progression in biological fluids include human serum samples and cancer cells. The nanoprobe also deliver clear signal to microRNA target in fixed and lived MDA-MB-231 cells. |
| Databáze: | OpenAIRE |
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