Intestinal microbiome as a risk factor for urinary tract infections in children
| Autor: | Ari Turpeinen, Juha Jyrkäs, Marjo Renko, Terhi Tapiainen, Aleksi Husso, Matti Uhari, Pirjo Koivusaari, Anna Maria Pirttilä, Niko Paalanne, Sampo Mattila, Oskari Pieviläinen, Tytti Pokka, Mysore V. Tejesvi, Jarmo Salo |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) medicine.medical_specialty Iron Biology Microbiology Gastroenterology Feces 03 medical and health sciences 0302 clinical medicine Medical microbiology Risk Factors RNA Ribosomal 16S 030225 pediatrics Internal medicine Escherichia coli medicine Humans Prospective Studies Microbiome Risk factor Lactoferrin Gastrointestinal Microbiome Case-control study Infant General Medicine Enterobacter biology.organism_classification 030104 developmental biology Infectious Diseases Case-Control Studies Child Preschool Urinary Tract Infections biology.protein Female |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 37:1881-1891 |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/s10096-018-3322-7 |
| Popis: | As urinary tract infection (UTI) pathogens originate from the gut, we hypothesized that the gut environment reflected by intestinal microbiome influences the risk of UTI. Our prospective case-control study compared the intestinal microbiomes of 37 children with a febrile UTI with those of 69 healthy children. We sequenced the regions of the bacterial 16S rRNA gene and used the LefSe algorithm to calculate the size of the linear discriminant analysis (LDA) effect. We measured fecal lactoferrin and iron concentrations and quantitative PCR for Escherichia coli. At the phylum level, there were no significant differences. At the genus level, Enterobacter was more abundant in UTI patients with an LDA score > 3 (log 10), while Peptostreptococcaceae were more abundant in healthy subjects with an LDA score > 3 (log 10). In total, 20 OTUs with significantly different abundances were observed. Previous use of antimicrobials did not associate with intestinal microbiome. The relative abundance of E. coli was 1.9% in UTI patients and 0.5% in controls (95% CI of the difference—0.8 to 3.6%). The mean concentration of E.coli in quantitative PCR was 0.14 ng/μl in the patients and 0.08 ng/μl in the controls (95% CI of the difference—0.04 to 0.16). Fecal iron and lactoferrin concentrations were similar between the groups. At the family and genus level, we noted several differences in the intestinal microbiome between children with UTI and healthy children, which may imply that the gut environment is linked with the risk of UTI in children. |
| Databáze: | OpenAIRE |
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