Dicer-2-Dependent Generation of Viral DNA from Defective Genomes of RNA Viruses Modulates Antiviral Immunity in Insects
| Autor: | Louis Lambrechts, Maria-Carla Saleh, Sophie Lanciano, Marco Vignuzzi, Hyelee Loyd, Lorena Tomé-Poderti, Hervé Blanc, Valérie Gausson, Enzo Z. Poirier, Bertsy Goic, Lionel Frangeul, Thomas Vallet, Laura I. Levi, Sarah H. Merkling, Susan Carpenter, Marie Mirouze, Chloé Baron, Jeremy Boussier |
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| Přispěvatelé: | Virus et Interférence ARN - Viruses and RNA Interference, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Populations virales et Pathogenèse - Viral Populations and Pathogenesis, Cellule Pasteur, Université Paris Diderot - Paris 7 (UPD7)-PRES Sorbonne Paris Cité, Immunobiologie des Cellules dendritiques, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Iowa State University (ISU), Diversité, adaptation, développement des plantes (UMR DIADE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Work was supported by the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID to L.L., M.V., and M.-C.S.), the European Research Council (FP7/2013-2019 ERC CoG 615220 to M.-C.S.), and the DARPA INTERCEPT program managed by Dr. Jim Gimlett and administered though DARPA Cooperative Agreement #HR0011-17-2-0023 to M.V. and M.-C.S. (the content of the information does not necessarily reflect the position or the policy of the U.S. government, and no official endorsement should be inferred). L.L. was supported by the City of Paris Emergence(s) program in Biomedical Research (grant DASES 2013-33) and by the European Union’s Horizon 2020 research and innovation program under ZikaPLAN grant agreement No. 734584. L.T.-P. is a Marie Skłodowska-Curie actions fellow (H2020 MSCA-IF 656398-DDRR)., We thank the Saleh and Vignuzzi labs, especially V. Mongelli, L. Carrau, and G. Moratorio for scientific advice, and B. tenOever for discussions. We thank Jean-Luc Imler for the various fly strains. We thank Catherine Lallemand for mosquito rearing and Alongkot Ponlawat for field collection of mosquitoes, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 615220,EC:FP7:ERC,ERC-2013-CoG,RNAIMMUNITY(2015), European Project: 734584,ZikaPLAN, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Ribonuclease III Small interfering RNA Genes Viral MESH: Drosophila [SDV]Life Sciences [q-bio] Virus Replication Dicer-2 DEAD-box RNA Helicases RNA Virus Infections MESH: Ribonuclease III RNA interference MESH: Arboviruses MESH: RNA Small Interfering Drosophila Proteins MESH: Animals RNA Small Interfering MESH: Genes Viral biology MESH: RNA Helicases persistence Viral Load 3. Good health Cell biology MESH: RNA Viral Host-Pathogen Interactions [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology RNA Viral [SDV.IMM]Life Sciences [q-bio]/Immunology Drosophila RNA Interference MESH: Genome Viral MESH: Viral Load MESH: RNA Viruses RNA Helicases MESH: Antiviral Agents RNA virus MESH: Drosophila Proteins MESH: RNA Interference Genome Viral Microbiology Antiviral Agents Virus 03 medical and health sciences Virology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Animals Point Mutation RNA Viruses MESH: DEAD-box RNA Helicases [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Point Mutation 030102 biochemistry & molecular biology MESH: RNA Virus Infections fungi MESH: Virus Replication MESH: Host-Pathogen Interactions circular viral DNA RNA [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology biology.organism_classification Reverse transcriptase MESH: DNA Viral 030104 developmental biology Culicidae arbovirus Viral replication RNAi DNA Viral biology.protein defective viral genomes Parasitology insect MESH: Culicidae Arboviruses Dicer |
| Zdroj: | Cell Host and Microbe Cell Host and Microbe, 2018, 23 (3), pp.353-365.e8. ⟨10.1016/j.chom.2018.02.001⟩ Cell Host & Microbe Cell Host and Microbe, Elsevier, 2018, 23 (3), pp.353-365.e8. ⟨10.1016/j.chom.2018.02.001⟩ |
| ISSN: | 1931-3128 |
| Popis: | International audience; The RNAi pathway confers antiviral immunity in insects. Virus-specific siRNA responses are amplified via the reverse transcription of viral RNA to viral DNA (vDNA). The nature, biogenesis, and regulation of vDNA are unclear. We find that vDNA produced during RNA virus infection of Drosophila and mosquitoes is present in both linear and circular forms. Circular vDNA (cvDNA) is sufficient to produce siRNAs that confer partially protective immunity when challenged with a cognate virus. cvDNAs bear homology to defective viral genomes (DVGs), and DVGs serve as templates for vDNA and cvDNA synthesis. Accordingly, DVGs promote the amplification of vDNA-mediated antiviral RNAi responses in infected Drosophila. Furthermore, vDNA synthesis is regulated by the DExD/H helicase domain of Dicer-2 in a mechanism distinct from its role in siRNA generation. We suggest that, analogous to mammalian RIG-I-like receptors, Dicer-2 functions like a pattern recognition receptor for DVGs to modulate antiviral immunity in insects. |
| Databáze: | OpenAIRE |
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