The gut microbiota modulates host amino acid and glutathione metabolism in mice
| Autor: | Cheng Cheng Zhang, Mattias Bergentall, Fredrik Bäckhed, Erik Larsson, Saeed Shoaie, Adil Mardinoglu, Jens Nielsen, Pouyan Ghaffari |
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| Rok vydání: | 2015 |
| Předmět: |
Male
genome-scale metabolic models Adipose tissue Ileum White adipose tissue Gut flora digestive system General Biochemistry Genetics and Molecular Biology Jejunum Mice transcriptomics 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine germ-free mice Intestine Small Journal Article medicine Animals Amino Acids 030304 developmental biology 0303 health sciences General Immunology and Microbiology biology Microbiota Research Support Non-U.S. Gov't Applied Mathematics Biochemistry and Molecular Biology Articles glutathione metabolism Glutathione Metabolism biology.organism_classification metabolomics Small intestine Mice Inbred C57BL medicine.anatomical_structure Computational Theory and Mathematics chemistry Biochemistry General Agricultural and Biological Sciences Cell and Molecular Biology 030217 neurology & neurosurgery Information Systems |
| Zdroj: | Molecular Systems Biology (1744-4292) vol.11(2015) Mardinoglu, A, Bergentall, M, Ghaffari, P, Zhang, C, Larsson, E, Bäckhed, F, Nielsen, J & Shoaie, S 2015, ' The gut microbiota modulates host amino acid and glutathione metabolism in mice ', Molecular Systems Biology, vol. 11, no. 10, pp. 834 . https://doi.org/10.15252/msb.20156487 Molecular Systems Biology |
| ISSN: | 1744-4292 |
| DOI: | 10.15252/msb.20156487 |
| Popis: | The gut microbiota has been proposed as an environmental factor that promotes the progression of metabolic diseases. Here, we investigated how the gut microbiota modulates the global metabolic differences in duodenum, jejunum, ileum, colon, liver, and two white adipose tissue depots obtained from conventionally raised (CONV-R) and germ-free (GF) mice using gene expression data and tissue-specific genome-scale metabolic models (GEMs). We created a generic mouse metabolic reaction (MMR) GEM, reconstructed 28 tissue-specific GEMs based on proteomics data, and manually curated GEMs for small intestine, colon, liver, and adipose tissues. We used these functional models to determine the global metabolic differences between CONV-R and GF mice. Based on gene expression data, we found that the gut microbiota affects the host amino acid (AA) metabolism, which leads to modifications in glutathione metabolism. To validate our predictions, we measured the level of AAs and N-acetylated AAs in the hepatic portal vein of CONV-R and GF mice. Finally, we simulated the metabolic differences between the small intestine of the CONV-R and GF mice accounting for the content of the diet and relative gene expression differences. Our analyses revealed that the gut microbiota influences host amino acid and glutathione metabolism in mice. |
| Databáze: | OpenAIRE |
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