Acute cylindrospermopsin exposure: Pulmonary and liver harm and mitigation by dexamethasone
| Autor: | Milena Simões Peixoto, Mariana Nascimento Machado, Christina Maeda Takiya, Priscila da Penha Apolinário Barboza, Dayene de Assis Ferenandes Caldeira, Luis F. Cruz, Mariana Abreu, Rodrigo S. Fortunato, Alysson R. Carvalho, Walter A. Zin |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Male medicine.medical_treatment Reversion Anti-Inflammatory Agents Inflammation Pharmacology Toxicology 01 natural sciences Dexamethasone 03 medical and health sciences chemistry.chemical_compound Mice Airway resistance Alkaloids Medicine Animals Saline Lung 0303 health sciences Mice Inbred BALB C Cyanobacteria Toxins business.industry 010604 marine biology & hydrobiology 030302 biochemistry & molecular biology medicine.disease Respiratory Function Tests medicine.anatomical_structure chemistry Liver Cylindrospermopsin medicine.symptom business Infiltration (medical) medicine.drug |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 191 |
| ISSN: | 1879-3150 |
| Popis: | Cylindrospermopsin (CYN) is a cyanotoxin of increasing worldwide environmental importance as it can harm human beings. Dexamethasone is a steroidal anti-inflammatory agent. Thus, we aimed at evaluating the pulmonary outcomes of acute CYN intoxication and their putative mitigation by dexamethasone. Male BALB/c mice received intratracheally a single dose of saline or CYN (140 μg/kg). Eighteen hours after exposure, mice instilled with either saline solution (Ctrl) or CYN were intramuscularly treated with saline (Tox) or 2 mg/kg dexamethasone (Tox + dexa) every 6 h for 48 h. Pulmonary mechanics was evaluated 66 h after instillation using the forced oscillation technique (flexiVent) to determine airway resistance (RN), tissue viscance (G) and elastance (H). After euthanasia, the lungs were removed and separated for quantification of CYN, myeloperoxidase activity and IL-6 and IL-17 levels plus histological analysis. CYN was also measured in the liver. CYN increased G and H, alveolar collapse, PMN cells infiltration, elastic and collagen fibers, activated macrophages, peroxidase activity in lung and hepatic tissues, as well as IL-6 and IL-17 levels in the lung. Tox + Dexa mice presented total or partial reversion of the aforementioned alterations. Briefly, CYN impaired pulmonary and hepatic characteristics that were mitigated by dexamethasone. |
| Databáze: | OpenAIRE |
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