Enzymatic production of low-Mw chitosan-derivatives: Characterization and biological activities evaluation
| Autor: | Hana Maalej, Angeles Heras, Niuris Acosta, Moncef Nasri, Inmaculada Aranaz, Sawsan Affes |
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| Rok vydání: | 2020 |
| Předmět: |
Chemical Phenomena
Glycoside Hydrolases Starch 02 engineering and technology Biochemistry Antioxidants Chitosan 03 medical and health sciences chemistry.chemical_compound Hydrolysis Bacterial Proteins Structural Biology Bacillus licheniformis Chitosanase Food science Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Chemistry Depolymerization General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Anti-Bacterial Agents Molecular Weight carbohydrates (lipids) Enzyme Tryptone 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 144:279-288 |
| ISSN: | 0141-8130 |
| Popis: | This study reports the enzymatic depolymerization of chitosan by the chitosanolytic preparation of Bacillus licheniformis GA11. For this purpose, chitosanase production and biochemical characterization of the crude enzyme preparation from GA11 were firstly investigated. The highest chitosanase production was obtained in a culture medium containing 50.0 g/l of a mixture of soluble starch/shrimp shells/crab shells and 5.0 g/l tryptone, after incubation during 5 days, at 30 °C with a pHi of 6.0 and under continuous agitation at 200 rpm. Then, the chitosanolytic preparation, exhibiting maximum activity at 65 °C and pH 5.0, was used to hydrolyze chitosan, leading to various chitosan-depolymerization products (CDP) with different physicochemical characteristics. Finally, the antioxidant and antimicrobial potentials of CDP were evaluated, allowing to conclude that the molecular weight and the acetylation degree highly affect the biological activities of CDP. |
| Databáze: | OpenAIRE |
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