Molecular characterization of Giardia lamblia in children less than 5 years of age with diarrhoea attending the Bengo General Hospital, Angola
| Autor: | Maria Clara Mirante, Daniela Portugal-Calisto, Ana Santos-Reis, Filipa Santana Ferreira, António Carlos Mayer, Susana Vaz Nery, Carolina Gasparinho, Miguel Brito |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Diarrhea Giardiasis Male Genotyping Sequence analysis 030231 tropical medicine Biology medicine.disease_cause Hospitals General Polymerase Chain Reaction DNA sequencing Microbiology law.invention 03 medical and health sciences Feces Hospital 0302 clinical medicine law parasitic diseases medicine Giardia lamblia Assemblage (archaeology) Humans Children Polymerase chain reaction Molecular Epidemiology Molecular epidemiology Província do Bengo Public Health Environmental and Occupational Health Infant Newborn Infant General Medicine Sequence Analysis DNA Original Articles DNA Protozoan Diarrhoea 3. Good health 030104 developmental biology Infectious Diseases Cross-Sectional Studies Angola GenBank Child Preschool Parasitology Female |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Introduction - Giardia lamblia is a pathogenic intestinal protozoan with high prevalence in developing countries, especially among children. Molecular characterization has revealed the existence of eight assemblages, with A and B being more commonly described in human infections. Despite its importance, to our knowledge, this is the first published molecular analysis of G. lamblia assemblages in Angola. Methods - The present study aimed to identify the assemblages of G. lamblia in children with acute diarrhoea presenting at the Bengo General Hospital, Angola. A stool sample was collected and microscopy and immunochromatographic tests were used. DNA was extracted and assemblage determination was performed through amplification of the gene fragment ssu-rRNA (175 bp) and β-giardin (511 bp) through polymerase chain reaction and DNA sequencing. Results - Of the 16 stool samples screened, 12 were successfully sequenced. Eleven isolates were assigned to assemblage B and one to assemblage A. Subassemblage determination was not possible for assemblage B, while the single isolate assigned to assemblage A was identified as belonging to subassemblage A3. Conclusion - This study provides information about G. lamblia assemblages in Bengo Province, Angola and may contribute as a first step in understanding the molecular epidemiology of this protozoan in the country. GenBank accession numbers for the ssur-RNA gene: MF479750, MF479751, MF479752, MF479753, MF479754, MF479755, MF479756, MF479757, MF479758, MF479759, MF479760, MF479761. GenBank accession numbers for the β-giardin gene: MF565378, MF565379, MF565380, MF565381. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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