The effect of the microstructure of β-tricalcium phosphate on the metabolism of subsequently formed bone tissue
| Autor: | Yasuaki Shibata, Hisashi Murayama, Shimeru Kamihira, Giichiro Kawachi, Koji Ioku, Hideyuki Minagi, Ikuho Yonezawa, Yoshinori Gonda, Takatoshi Okuda, Hisashi Kurosawa, Soichiro Minami, Tohru Ikeda |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Calcium Phosphates
Materials science Biophysics Osteoclasts chemistry.chemical_element Biocompatible Materials Bioengineering Calcium Bone tissue Biomaterials Implants Experimental Osteogenesis Osteoclast Materials Testing Bone cell medicine Animals Femur Osteoblast Anatomy Resorption medicine.anatomical_structure chemistry Mechanics of Materials Bone Substitutes Ceramics and Composites Female Hydroxyapatites Rabbits Bone marrow |
| Zdroj: | Biomaterials. 28:2612-2621 |
| ISSN: | 0142-9612 |
| Popis: | The response of bone cells to a newly developed porous beta-tricalcium phosphate composed of rod-shaped particles (RSbeta-TCP), beta-TCP composed of conventional non-rod-shaped particles (Cbeta-TCP), and hydroxyapatite (HA) was analyzed using in vivo implantation and in vitro osteoclastogenesis systems. Implantation of the materials into the rabbit femur showed that RSbeta-TCP and Cbeta-TCP were bioresorbable, but HA was not. Up to 12 weeks after the implantation, bioresorption of RSbeta-TCP and Cbeta-TCP accompanied by the formation of new bone occurred satisfactorily. At 24 weeks post-implantation, most of the RSbeta-TCP had been absorbed, and active osteogenesis was preserved in the region. However, in the specimens implanted with Cbeta-TCP, the amount of not only the implanted Cbeta-TCP but also the newly formed bone tissue decreased, and bone marrow dominated the region. The implanted HA was unbioresorbable throughout the experimental period. When osteoclasts were generated on RSbeta-TCP, Cbeta-TCP, or HA disks, apparent resorption lacunae were formed on the RSbeta-TCP and Cbeta-TCP, but not HA disks. Quantitation of the calcium concentration in the culture media showed an earlier and more constant release of calcium from RSbeta-TCP than Cbeta-TCP. These results showed that the microstructure of beta-TCP affects the activity of bone cells and subsequent bone replacement. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect