Regeneration of Algerian Citrus germplasm by stigma/style somatic embryogenesis
| Autor: | Meziane M, Boudjeniba M, Frasheri D, D'Onghia AM, Carra A, Carimi F, Haddad N, Boukhalfa S, Braneci S. |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | African journal of biotechnology 11 (2012): 6666–6672. doi:10.5897/AJB10.2485 info:cnr-pdr/source/autori:Meziane M, Boudjeniba M, Frasheri D, D'Onghia AM, Carra A, Carimi F, Haddad N, Boukhalfa S, Braneci S/titolo:Regeneration of Algerian Citrus germplasm by stigma%2Fstyle somatic embryogenesis/doi:10.5897%2FAJB10.2485/rivista:African journal of biotechnology/anno:2012/pagina_da:6666/pagina_a:6672/intervallo_pagine:6666–6672/volume:11 African journal of biotechnology (2012): 25–6666. info:cnr-pdr/source/autori:Meziane M, Boudjeniba M, Frasheri D, D'Onghia AM, Carra A, Carimi F, Haddad N, Boukhalfa S, Braneci S./titolo:Regeneration of Algerian Citrus germplasm by stigma%2Fstyle somatic embryogenesis./doi:/rivista:African journal of biotechnology/anno:2012/pagina_da:25/pagina_a:6666/intervallo_pagine:25–6666/volume |
| DOI: | 10.5897/AJB10.2485 |
| Popis: | Stigma/style somatic embryogenesis is one of the efficient methods in plant regeneration of most Citrus ssp., without inducing somaclonal variations. Furthermore, somatic embryogenesis from style/stigma proved to be effective in the elimination of the main citrus virus and virus-like diseases. This technique was applied on Algerian citrus collection. Different Citrus species [Citrus sinensis (L.) Osbeck, C. limon (L.) Burm, C. reticulata Blanco, C. paradisi Macfad, C. reshni Hort. ex Tan., C. jambhiri Lush and C. maxima (Burm.) Merrill] were chosen and tested for the presence of the main virus and virus-like agents. Most of the genotypes showed to be infected, mainly by viroid agents. Closed flowers were collected and in vitro cultured on a Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine. All explants produced callus about 4 to 9 days after culture initiation, whereas embryogenesis occurred after 38 to 150 days in most of the cultured genotypes. Formed embryos were cultured in a single tube before in vivo acclimatization. After sanitary assays, regenerated plants were shown to be free from the agents detected in the mother trees. |
| Databáze: | OpenAIRE |
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