Small-angle neutron and X-ray scattering analysis of the supramolecular organization of rhodopsin in photoreceptor membrane
| Autor: | T. B. Feldman, Adam Round, Mikhail A. Ostrovsky, A.V. Belushkin, T. N. Murugova, Irina B. Kolchugina, Oleksandr I. Ivankov, M. A. Yakovleva, Valentin Gordeliy, Alexander I. Kuklin, O. A. Smitienko |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Rhodopsin genetic structures Dimer Biophysics Supramolecular chemistry 010402 general chemistry 01 natural sciences Biochemistry Retina 03 medical and health sciences chemistry.chemical_compound Scattering Small Angle Molecule Animals Neutron Photoreceptor Cells Neutrons Membranes biology Chemistry Scattering Cell Membrane Cell Biology eye diseases 0104 chemical sciences Neutron Diffraction 030104 developmental biology Monomer Membrane biology.protein Cattle sense organs |
| Zdroj: | Biochimica et biophysica acta. Biomembranes. 1861(10) |
| ISSN: | 1879-2642 |
| Popis: | The supramolecular organization of the visual pigment rhodopsin in the photoreceptor membrane remains contentious. Specifically, whether this G protein-coupled receptor functions as a monomer or dimer remains unknown, as does the presence or absence of ordered packing of rhodopsin molecules in the photoreceptor membrane. Completely opposite opinions have been expressed on both issues. Herein, using small-angle neutron and X-ray scattering approaches, we performed a comparative analysis of the structural characteristics of the photoreceptor membrane samples in buffer, both in the outer segment of photoreceptor cells, and in the free photoreceptor disks. The average distance between the centers of two neighboring rhodopsin molecules was found to be ~5.8 nm in both cases. The results indicate an unusually high packing density of rhodopsin molecules in the photoreceptor membrane, but molecules appear to be randomly distributed in the membrane without any regular ordering. |
| Databáze: | OpenAIRE |
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