Prostacyclin receptor signaling and early embryo development in the mouse
| Autor: | Jennifer S. Goldsby, Kenneth K. Wu, Karine Egan, Garret A. FitzGerald, Wan-Song A. Wun, Jaou-Chen Huang |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty animal structures Embryonic Development Mice Transgenic Prostacyclin Biology Receptors Epoprostenol Mice Pregnancy Internal medicine medicine Animals Embryo Implantation Blastocyst Receptor Prostaglandin receptor Prostacyclin receptor Mice Inbred C3H Rehabilitation Embryogenesis Gene Expression Regulation Developmental Obstetrics and Gynecology Embryo Epoprostenol Mice Inbred C57BL medicine.anatomical_structure Endocrinology Reproductive Medicine embryonic structures Oocytes Pregnancy Animal Female lipids (amino acids peptides and proteins) Peroxisome proliferator-activated receptor delta Signal Transduction medicine.drug |
| Zdroj: | Human Reproduction. 22:2851-2856 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/humrep/dem304 |
| Popis: | Background Prostacyclin (PGI(2)) plays an important role in mouse embryo development and implantation. However, it is unclear whether its action is mediated via the I prostaglandin receptor (IP). Methods We compared the preimplantation development of IP deleted (IP-/-) embryos and wild-type (WT) embryos. We also evaluated the effect of iloprost, a stable PGI(2) analog, and L-165041, a peroxisome proliferator activated receptor delta (PPARdelta) ligand, on IP-/- versus WT embryos. Finally, we compared the development of heterozygous IP deficient embryos carrying a normal maternal IP allele versus paternal IP allele. Results Development of IP-/- embryos lagged behind WT embryos and was not enhanced by either the PGI(2) analog or the PPARdelta ligand. WT embryos had slightly higher, although statistically not significant, implantation rates than IP-/- embryos. Heterozygous IP deficient embryos carrying a normal maternal IP allele showed better development and responded to the PGI(2) analog, unlike those carrying the normal paternal IP allele. Conclusions IP receptors play an important role in preimplantation embryo development and mediate the embryo's response to exogenous PGI(2). Early embryo development depends on the oocyte IP receptor. |
| Databáze: | OpenAIRE |
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