Construction and analysis of a subtractive cDNA library of early embryonic development in duck
| Autor: | L.X. Zhong, Z.R. Tao, Fangxiong Shi, D.Q. Wang, Jian Li, L.Z. Lu, Y. L. Liu, J.D. Shen |
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| Rok vydání: | 2013 |
| Předmět: |
Homeodomain Proteins
Homeobox protein NANOG cDNA library Molecular Sequence Data Embryogenesis Gene Expression Regulation Developmental General Medicine Biology Embryonic stem cell Cell biology Ducks Transcription (biology) Complementary DNA Gene expression Genetics Animals Amino Acid Sequence Molecular Biology Gene Gene Library |
| Zdroj: | Genetics and Molecular Research. 12:2234-2247 |
| ISSN: | 1676-5680 |
| Popis: | Several studies have documented the process of early embryonic development in poultry; however, the molecular mechanisms underlying its developmental regulation are poorly understood, particularly in ducks. In this study, we analyzed differential gene expression of embryos 6 and 25 h following oviposition to determine which genes regulate the early developmental stage in ducks. Among 216 randomly selected clones, 39 protein-encoding cDNAs that function in metabolism, transcription, transportation, proliferation/apoptosis, cell cycle, cell adhesion, and methylation were identified. Additionally, the full-length cDNA of the Nanog gene, encoding a 302-amino acid protein, was obtained. Quantitative real-time polymerase chain reaction analyses were performed to detect expression levels of the selected genes during early and late embryonic stages, which revealed that these genes are expressed in a particular spatial and temporal pattern. These results indicate that these genes may play pivotal roles in the process of area pellucida formation through a complex and precise regulatory network during development in duck embryos. |
| Databáze: | OpenAIRE |
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