Extracts of endophytic fungi from leaves of selected Nigerian ethnomedicinal plants exhibited antioxidant activity
| Autor: | Mutiat B. Ibrahim, Elizabeth Olawumi Oyebanji, Chiamaka Orabuchi, Adedotun Adeyinka Adekunle, Babajide Akinnawo, Muinah A Fowora, Ayobami Aiyeolemi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Alchornea cordifolia
DPPH Free radical scavenging Endophytic fungi ved/biology.organism_classification_rank.species Nigeria Plant use of endophytic fungi in defense Antioxidants 03 medical and health sciences chemistry.chemical_compound Phytol 0302 clinical medicine Endophytes Gallic acid Nigerian plants 030304 developmental biology Lupeol 0303 health sciences Stigmasterol Plants Medicinal biology Traditional medicine ved/biology Secondary metabolites Fungi lcsh:Other systems of medicine biology.organism_classification lcsh:RZ201-999 Plant Leaves ITS-rDNA Ethnomedicinal plants Complementary and alternative medicine chemistry Gomphrena celosioides 030220 oncology & carcinogenesis Research Article |
| Zdroj: | BMC Complementary Medicine and Therapies, Vol 21, Iss 1, Pp 1-13 (2021) BMC Complementary Medicine and Therapies |
| ISSN: | 2662-7671 |
| Popis: | Background Plants with an ethnobotanical history are known to harbor diverse group of endophytic fungi, which constitute major natural sources of bioactive compounds. In the present study, we evaluated the antioxidant activity of endophytic fungi from eight Nigerian ethnomedicinal plants. Endophytic fungi were isolated from the leaves of Acalypha ornata, Albizia zygia, Alchornea cordifolia, Chrysophyllum albidum, Ficus exasperata, Gomphrena celosioides, Millettia thonningii, and Newbouldia laevis. Methods Endophytic fungi were isolated from the leaves of selected plants via surface sterilization. Isolated fungi were identified by internal transcribed spacer (ITS-rDNA) sequence analysis. Pure fungal strains were subjected to fermentation process on solid rice medium and metabolites extracted using ethyl-acetate. Fungal crude extracts were screened for antioxidant activity using 2, 2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reduction of ferric ion assays. Gas chromatography/mass spectrometry (GC/MS) analysis was used to identify the major chemical constituents in active fungal extracts. Results A total of eighteen fungal endophytes with fungal codes CU (061 and 062); ZA (161, 162, 163, and 164); LO (261); CA (041, 042, and 043); FE (081, 082, and 084); GE (091); MO (211 and 212); and NA (021 and 022) were isolated from the eight ethnomedicinal plants A. ornata, A. zygia, A. cordifolia, C. albidum, F. exasperata, G. celosioides, M. thonningii, and N. laevis respectively. ZA 163 and MO 211 fungal extracts showed significant (p 50 values of 50.53 ± 0.01 and 86.69 ± 0.02 μg/ml respectively. Fungal extract CA 041 demonstrated significantly (p Conclusion These results showed that leaves of the selected Nigerian plants harbor diverse group of endophytic fungi, which can be potential antioxidant resource. Graphical abstract |
| Databáze: | OpenAIRE |
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