A radiometric assay for phenylethanolamine N-methyltransferase and catechol O-methyltransferase in a single tissue sample: Application to rat hypothalamic nuclei, pineal gland, and heart
| Autor: | Juraj Culman, Virginia K. Weise, Tichomir Torda |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
S-Adenosylmethionine endocrine system Hypothalamus Biophysics Catechol O-Methyltransferase Pineal Gland Biochemistry chemistry.chemical_compound Pineal gland medicine Animals Radiometry Molecular Biology Metanephrine chemistry.chemical_classification Methionine Catechol-O-methyl transferase biology Myocardium Phenylethanolamine N-Methyltransferase Rats Inbred Strains Cell Biology Enzyme assay Phenylethanolamine N-methyltransferase Rats Phenylethanolamine medicine.anatomical_structure Enzyme chemistry biology.protein |
| Zdroj: | Analytical Biochemistry. 164:345-354 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(87)90503-3 |
| Popis: | A simple and highly sensitive method for simultaneous assay of phenylethanolamine N-methyltransferase (PNMT) and catechol O-methyltransferase (COMT) is described. These enzymes are determined in a single tissue homogenate using S-[methyl-3H]adenosyl- l -methionine as methyl donor and sequentially incubating with the substrates phenylethanolamine and epinephrine. The radioactive products of the enzymatic reactions, N-methylphenylethanolamine and metanephrine, are extracted and then separated by thin-layer chromatography. The identity of the reaction products has been established chromatographically and the conditions for both enzymatic reactions in the assay procedure have been defined. Measurement of PNMT activity in the rat pineal gland or in minute fragments of other tissues (e.g., brain nuclei) has not been possible using previously described methods. Activities of PNMT and COMT in the rat pineal gland, various hypothalamic nuclei, and the auricular and ventricular myocardia are herein reported. |
| Databáze: | OpenAIRE |
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