Genotoxicity biomarkers in the amphipod Gammarus elvirae exposed in vivo to mercury and lead and basal levels of DNA damage in two cell types
| Autor: | E. De Matthaeis, Lucilla Ronci, G. Di Donato, Andrea Setini |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell type DNA damage chemistry.chemical_element 010501 environmental sciences Biology medicine.disease_cause 01 natural sciences Toxicology 03 medical and health sciences In vivo medicine Gammarus elvirae Ecology Evolution Behavior and Systematics 0105 earth and related environmental sciences General Environmental Science Ecology Heavy metals genotoxicity biomarkers Amphipoda Mercury (element) Comet assay 030104 developmental biology chemistry Biochemistry General Earth and Planetary Sciences Genotoxicity |
| Popis: | The present work deals with the application of genotoxicity biomarkers by means of the Comet assay in haemocytes and spermatozoa of the crustacean Gammarus elvirae exposed in vivo to heavy metals. Furthermore, a basal levels (BLs) study of DNA damage in the two cell types considered for two different gammarids species, G. elvirae and Echinogammarus veneris, was carried out. It is important to identify factors that influence the outcome of the assay in order to obtain reliable and reproducible results usable for risk assessment purposes. Our results highlight that the Italian legal limits for Hg and Pb, respectively, 0.5 and 50 µg/L, are inadequate for establishing safety thresholds in the aquatic environment. Furthermore, the freshwater invertebrate G. elvirae, used for the first time to measure the effect of genotoxicants, is a good candidate for evaluating the genotoxicity damage induced by heavy metals. Our results concerning spermatozoa show excessively variable responses and high BLs. |
| Databáze: | OpenAIRE |
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