Acanthopanax senticosus Protects Structure and Function of Mesencephalic Mitochondria in A Mouse Model of Parkinson’s Disease
| Autor: | Zhi-ming yang, Chong-Zhi Wang, Xu-zhao Li, Shu-min Liu, Fang Lu, Ke-xin Wang, Chun-Su Yuan, Shuai-nan Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine SDHA Eleutherococcus Dehydrogenase Oxidative phosphorylation Nicotinamide adenine dinucleotide Mitochondrion Pharmacology Mice 03 medical and health sciences chemistry.chemical_compound Mesencephalon Animals Pharmacology (medical) Membrane Potential Mitochondrial chemistry.chemical_classification Reactive oxygen species biology Plant Extracts MPTP Succinate dehydrogenase MPTP Poisoning General Medicine Mice Inbred C57BL Neuroprotective Agents 030104 developmental biology Complementary and alternative medicine chemistry biology.protein Mitochondrial Swelling |
| Zdroj: | Chinese Journal of Integrative Medicine. 24:835-843 |
| ISSN: | 1993-0402 1672-0415 |
| Popis: | To investigate the neuro-protective effects of Acanthopanax senticosus Harms (EAS) on mesencephalic mitochondria and the mechanism of action, using a mouse model of Parkinson’s disease (PD). The chemical fingerprint analysis of the extract of Acanthopanax senticosus Harms (EAS) was performed using the ultra performance liquid chromatograph and time of flight mass spectrometry. Thirty mice were randomly divided into the control group, the MPTP model group, and the EAS treated group with MPTP (MPTP+EAS group, 10 in each group). The MPTP model group and the MPTP+EAS group received MPTP-HCl (30 mg/kg i.p) once a day for 5 days. The control group received an equal volume of saline (20 mL/kg i.p) once a day for 5 days. Induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine hydrochloride daily (MPTP-HCl, 30 mg/kg) for 5 days, the PD mice were treated with EAS at 45.5 mg/kg daily for 20 days. The behavioral testing of mice was carried out using the pole-climbing test. The integrity and functions of neurons were examined in mesencephalic mitochondria in a PD mouse model, including nicotinamide adenine dinucleotide dehydrogenase ubiquinone flavoprotein 2 (NDUFV2), mitochondrially encoded nicotinamide adenine dinucleotide dehydrogenase 1 (MT-ND1), succinate dehydrogenase complex subunit A (SDHA), and succinate dehydrogenase cytochrome b560 subunit (SDHC). After treatment with EAS, the behavioral changes induced by MPTP were attenuated significantly (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink