Selective and differential medium for recovery of Pseudomonas cepacia from the respiratory tracts of patients with cystic fibrosis
| Autor: | M J Marcon, John M. Matsen, B C Hilman, M J Muszynski, P A Ahlin, D F Welch, P H Gilligan, S A Chartrand, C H Pai, M M Hribar |
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| Rok vydání: | 1987 |
| Předmět: |
Microbiology (medical)
food.ingredient Cystic Fibrosis biology Respiratory System Pseudomonas Antimicrobial medicine.disease Isolation (microbiology) biology.organism_classification Cystic fibrosis Culture Media Microbiology chemistry.chemical_compound food chemistry medicine Humans bacteria Agar Respiratory system Lactose Sheep blood Research Article |
| Zdroj: | Journal of Clinical Microbiology. 25:1730-1734 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.25.9.1730-1734.1987 |
| Popis: | A selective and differential medium, OFPBL (oxidation-fermentation base supplemented with agar, lactose, and two antimicrobial agents), for the isolation of Pseudomonas cepacia from respiratory specimens of patients with cystic fibrosis was developed and tested. Among 725 specimens submitted from seven centers over a 4- to 6-month period, 58 (8%) yielded P. cepacia on OFPBL; only 19 of these were recovered on MacConkey or sheep blood agar (P less than 0.001). No isolate was recovered on MacConkey or sheep blood agar alone. Ranges of recovery rates among centers were 0 to 15% on OFPBL and 0 to 10% on MacConkey or sheep blood agar. Ninety percent of P. cepacia isolates were detected on OFPBL in less than or equal to 3 days. Other nonfermenters and yeasts isolated on OFPBL were distinguished from P. cepacia by failure to acidify the medium. The new medium was clearly superior to MacConkey and sheep blood agars for the isolation of P. cepacia from the respiratory secretions of patients with cystic fibrosis. |
| Databáze: | OpenAIRE |
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