Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations
| Autor: | Thiago M. Batista, Caio Suzart Argolo, Karina Solis Hidalgo, Edson Mario de Andrade Silva, Daniel Oliveira Jordão do Amaral, Glória Regina Franco, Karina Peres Gramacho, Enrique Arévalo-Gardini, Fabienne Micheli, Rute R. da Fonseca, Mariana Rocha de Carvalho, Mariana Araujo Barreto, Ceslaine Santos Barbosa, Carlos Priminho Pirovani |
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| Přispěvatelé: | CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
cacao fungal protein Moniliophthora fungal cell wall phylogeny Moniliophthora perniciosa Moniliophthora roreri F30 - Génétique et amélioration des plantes Mycoprotéine Peru Expression des gènes oxidative stress genetics DNA Fungal Solanaceae Phylogeny 2. Zero hunger Genetics Fungal protein whole genome sequencing Effector Pathogenicity factors Plant disease unclassified drug effectorome classification fungal genome Ecuador Genome Fungal Witches' broom Brazil Biotechnology Research Article lcsh:QH426-470 lcsh:Biotechnology Pouvoir pathogène Herrania proteome gene sequence Biology chemistry Article génomique Fungal Proteins 03 medical and health sciences Pathogenomics lcsh:TP248.13-248.65 controlled study Theobroma cacao H20 - Maladies des plantes plant disease Plant Diseases Cacao nonhuman fungal DNA Whole Genome Sequencing isolation and purification microbiology 15. Life on land biology.organism_classification lcsh:Genetics 030104 developmental biology fungal virulence Frosty pod rot Plant pathogens Agaricales metabolism Witches’ broom |
| Zdroj: | BMC Genomics Barbosa, C S, Rodrigues da Fonseca, R A, Batista, T M, Barreto, M A, Argolo, C S, de Carvalho, M R, Jordao do Amaral, D O, de Andrade Silva, E M, Arevalo-Gardini, E, Hidalgo, K S, Franco, G R, Pirovani, C P, Micheli, F & Gramacho, K P 2018, ' Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations ', BMC Genomics, vol. 19, 509 . https://doi.org/10.1186/s12864-018-4875-7 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP BMC Genomics, Vol 19, Iss 1, Pp 1-12 (2018) |
| ISSN: | 1471-2164 |
| Popis: | Background: The hemibiotrophic pathogens Moniliophthora perniciosa (witches' broom disease) and Moniliophthora roreri (frosty pod rot disease) are among the most important pathogens of cacao. Moniliophthora perniciosa has a broad host range and infects a variety of meristematic tissues in cacao plants, whereas M. roreri infects only pods of Theobroma and Herrania genera. Comparative pathogenomics of these fungi is essential to understand Moniliophthora infection strategies, therefore the detection and in silico functional characterization of effector candidates are important steps to gain insight on their pathogenicity. Results: Candidate secreted effector proteins repertoire were predicted using the genomes of five representative isolates of M. perniciosa subpopulations (three from cacao and two from solanaceous hosts), and one representative isolate of M. roreri from Peru. Many putative effectors candidates were identified in M. perniciosa: 157 and 134 in cacao isolates from Bahia, Brazil; 109 in cacao isolate from Ecuador, 92 and 80 in wild solanaceous isolates from Minas Gerais (Lobeira) and Bahia (Caiçara), Brazil; respectively. Moniliophthora roreri showed the highest number of effector candidates, a total of 243. A set of eight core effectors were shared among all Moniliophthora isolates, while others were shared either between the wild solanaceous isolates or among cacao isolates. Mostly, candidate effectors of M. perniciosa were shared among the isolates, whereas in M. roreri nearly 50% were exclusive to the specie. In addition, a large number of cell wall-degrading enzymes characteristic of hemibiotrophic fungi were found. From these, we highlighted the proteins involved in cell wall modification, an enzymatic arsenal that allows the plant pathogens to inhabit environments with oxidative stress, which promotes degradation of plant compounds and facilitates infection. Conclusions: The present work reports six genomes and provides a database of the putative effectorome of Moniliophthora, a first step towards the understanding of the functional basis of fungal pathogenicity. © 2018 The Author(s). This work was done in the frame of the International Consortium in Advanced Biology (CIBA; https://www.ciba-network.org). The authors thank the Molecular Plant Pathology Laboratory and the Plant Pathology Laboratory at INIAP personnel for their assistance in obtaining the DNAs, Dr Carmen Suarez Capello for her kind assistance in Ecuador, and the Núcleo de Biologia Computacional e Gestão de Informações Biotecnológicas - UESC (NBCGIB), and Copenhague University for providing bioinformatics facility. Data sets were processed in sagarana HPC cluster, CPAD-ICB-UFMG. The authors would also like to thank Dr. Claudia Fortes Ferreira (Embrapa CNPMF, Brazil) and Dr. Raul Renné Valle (CEPLAC/CEPEC, Brazil) for English language revision. We are also grateful to Ivanna Michelle Meraz Pérez for helping translating an early version of this manuscript and to the anonymous reviewers who provided helpful comments to our work. KPG, FM and CPP were supported by research fellowship Pq-1 from CNPq. National Council for Scientific Development (CNPq) n° 311759/2014–9. CSB acknowledges FAPESB (Foundation for Research Support of the State of Bahia) for supporting her with a research assistantship during her Master’s Programme. |
| Databáze: | OpenAIRE |
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