Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model

Autor: Laurent Goffin, Marc Aprahamian, Gaétan Bour, Jacques Gangloff, Bernard Bayle, Jean-Marc Egly, Fernand Martel, Jacques Marescaux
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Pathology
medicine.medical_specialty
Carcinoma
Hepatocellular
Clinical Research Design
Preclinical Models
Science
Gastroenterology and Hepatology
Research and Analysis Methods
Biochemistry
Mice
Fibrosis
Cell Line
Tumor
Gastrointestinal Cancers
Medicine and Health Sciences
medicine
Carcinoma
Animals
Doxorubicin
Animal Models of Disease
Cell Proliferation
Multidisciplinary
business.industry
Mechanical Engineering
Radiology and Imaging
Liver Neoplasms
Biology and Life Sciences
Cancer
Robotics
X-Ray Microtomography
medicine.disease
Xenograft Model Antitumor Assays
Mice
Inbred C57BL
Disease Models
Animal
Research Design
Hepatocellular carcinoma
Cancer cell
Drug delivery
Animal Studies
Drug Evaluation
Engineering and Technology
Medicine
business
Liver cancer
Biomarkers
Research Article
medicine.drug
Zdroj: PLoS ONE, Vol 9, Iss 9, p e106675 (2014)
PLoS ONE
ISSN: 1932-6203
Popis: Hepatocellular carcinoma (HCC) is one of the most common cancer related deaths worldwide. One of the main challenges in cancer treatment is drug delivery to target cancer cells specifically. Preclinical evaluation of intratumoral drugs in orthotopic liver cancer mouse models is difficult, as percutaneous injection hardly can be precisely performed manually. In the present study we have characterized a hepatoma model developing a single tumor nodule by implantation of Hep55.1C cells in the liver of syngeneic C57BL/6J mice. Tumor evolution was followed up by µCT imaging, and at the histological and molecular levels. This orthotopic, poorly differentiated mouse HCC model expressing fibrosis, inflammation and cancer markers was used to assess the efficacy of drugs. We took advantage of the high precision of a previously developed robotized system for automated, image-guided intratumoral needle insertion, to administer every week in the tumor of the Hep55.1C mouse model. A significant tumor growth inhibition was observed using our robotized system, whereas manual intraperitoneal administration had no effect, by comparison to untreated control mice.
Databáze: OpenAIRE
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