Partial characterization of the adsorbed protein layer on Dirofilaria immitis (Nematoda) cuticle
| Autor: | A. K. Kadipasaoglu, F. H. Bilge |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Pathology
medicine.medical_specialty Cuticle Dirofilaria immitis Arthropod cuticle Biology law.invention Immunolabeling law medicine Animals Filarioidea General Veterinary Membrane Proteins General Medicine biology.organism_classification Immunohistochemistry Microscopy Electron Infectious Diseases Membrane Transmission electron microscopy Insect Science Biophysics Parasitology Female Electron microscope Layer (electronics) |
| Zdroj: | Parasitology research. 75(7) |
| ISSN: | 0932-0113 |
| Popis: | Electron microscopy coupled with ferritin-conjugated indirect immunolabeling was used to locate sites of adsorbed host protein on cuticular surfaces of the adult canine heartworm, Dirofilaria immitis. The epicuticle appeared as a trilaminated structure. At high magnifications, the outermost layer of this structure was resolved into a trilaminar layer, which might correspond to the plasma membrane of animal cells. A ruthenium red-positive layer was external to the epicuticle. Ferritin-antibody conjugates showed evidence of adsorbed dog albumin, dog immunoglobulin class G (IgG), and dog complement fraction 3 (C3) on the surface. Ferritin adsorption to control surfaces was minimal. Possible causes and effects of interfacial host-protein adsorption are discussed in an attempt to bring insight to the hemocompatible nature of the parasitic cuticle. |
| Databáze: | OpenAIRE |
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